論文

査読有り
2013年9月

Affinity purification and characterization of a biodegradable plastic-degrading enzyme from a yeast isolated from the larval midgut of a stag beetle, Aegus laevicollis

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Ken Suzuki
  • ,
  • Hironori Sakamoto
  • ,
  • Yukiko Shinozaki
  • ,
  • Jun Tabata
  • ,
  • Takashi Watanabe
  • ,
  • Atsushi Mochizuki
  • ,
  • Motoo Koitabashi
  • ,
  • Takeshi Fujii
  • ,
  • Seiya Tsushima
  • ,
  • Hiroko K. Kitamoto

97
17
開始ページ
7679
終了ページ
7688
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00253-012-4595-x
出版者・発行元
SPRINGER

Two yeast strains, which have the ability to degrade biodegradable plastic films, were isolated from the larval midgut of a stag beetle, Aegus laevicollis. Both of them are most closely related to Cryptococcus magnus and could degrade biodegradable plastic (BP) films made of poly(butylene succinate) (PBS) and poly(butylene succinate-co-adipate) (PBSA) effectively. A BP-degrading enzyme was purified from the culture broth of one of the isolated strains employing a newly developed affinity purification method based on the binding action of the enzyme to the substrate (emulsified PBSA) and its subsequent degradative action toward the substrate. Partial amino acid sequences of this enzyme suggested that it belongs to the cutinase family, and thus, the enzyme was named CmCut1. It has a molecular mass of 21 kDa and a degradative activity for emulsified PBSA which was significantly enhanced by the simultaneous presence of Ca2+ or Mg2+ at a concentration of about 2.5 mM. Its optimal pH was 7.5, and the optimal temperature was 40 A degrees C. It showed a broad substrate specificity for p-nitrophenyl (pNP)-fatty acid esters ranging from pNP-acetate (C2) to pNP-stearate (C18) and films of PBSA, PBS, poly(epsilon-caprolactone), and poly(lactic acid).

Web of Science ® 被引用回数 : 20

リンク情報
DOI
https://doi.org/10.1007/s00253-012-4595-x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23224497
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000323281900015&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00253-012-4595-x
  • ISSN : 0175-7598
  • PubMed ID : 23224497
  • Web of Science ID : WOS:000323281900015

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