論文

査読有り
2013年4月

Biodegradable plastic-degrading enzyme from Pseudozyma antarctica: cloning, sequencing, and characterization

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Yukiko Shinozaki
  • ,
  • Tomotake Morita
  • ,
  • Xiao-hong Cao
  • ,
  • Shigenobu Yoshida
  • ,
  • Motoo Koitabashi
  • ,
  • Takashi Watanabe
  • ,
  • Ken Suzuki
  • ,
  • Yuka Sameshima-Yamashita
  • ,
  • Toshiaki Nakajima-Kambe
  • ,
  • Takeshi Fujii
  • ,
  • Hiroko K. Kitamoto

97
7
開始ページ
2951
終了ページ
2959
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00253-012-4188-8
出版者・発行元
SPRINGER

Pseudozyma antarctica JCM 10317 exhibits a strong degradation activity for biodegradable plastics (BPs) such as agricultural mulch films composed of poly(butylene succinate) (PBS) and poly(butylene succinate-co-adipate) (PBSA). An enzyme named PaE was isolated and the gene encoding PaE was cloned from the strain by functional complementation in Saccharomyces cerevisiae. The deduced amino acid sequence of PaE contains 198 amino acids with a predicted molecular weight of 20,362.41. High identity was observed between this sequence and that of cutinase-like enzymes (CLEs) (61-68 %); therefore, the gene encoding PaE was named PaCLE1. The specific activity of PaE against emulsified PBSA was 54.8 +/- 6.3 U/mg. In addition to emulsified BPs, PaE degraded solid films of PBS, PBSA, poly(epsilon-caprolactone), and poly(lactic acid).

Web of Science ® 被引用回数 : 66

リンク情報
DOI
https://doi.org/10.1007/s00253-012-4188-8
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22678026
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000316481800017&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00253-012-4188-8
  • ISSN : 0175-7598
  • PubMed ID : 22678026
  • Web of Science ID : WOS:000316481800017

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