論文

査読有り
2011年11月

Silage produces biofuel for local consumption

BIOTECHNOLOGY FOR BIOFUELS
  • Hiroko K. Kitamoto
  • ,
  • Mitsuo Horita
  • ,
  • Yimin Cai
  • ,
  • Yukiko Shinozaki
  • ,
  • Keiji Sakaki

4
開始ページ
46
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/1754-6834-4-46
出版者・発行元
BIOMED CENTRAL LTD

Background: In the normal process of bioethanol production, biomass is transported to integrated large factories for degradation to sugar, fermentation, and recovery of ethanol by distillation. Biomass nutrient loss occurs during preservation and degradation. Our aim was to develop a decentralized ethanol production system appropriate for farm or co-operative level production that uses a solid-state fermentation method for producing bio-ethanol from whole crops, provides cattle feed, and produces no wastes. The idea is to incorporate traditional silage methods with simultaneous saccharification and fermentation. Harvested, fresh biomass is ensiled with biomass-degrading enzymes and yeast. Multiple parallel reactions for biomass degradation and ethanol and lactic acid production are induced in solid culture in hermetically sealed containers at a ranch. After fermentation, ethanol is collected on site from the vapor from heated fermented products.
Results: The parallel reactions of simultaneous saccharification and fermentation were induced efficiently in the model fermentation system. In a laboratory-scale feasibility study of the process, 250 g of freshly harvested forage rice with 62% moisture was treated with 0.86 filter paper units/g dry matter (DM) of cellulase and 0.32 U/g DM of glucoamylase. After 20 days of incubation at 28 C, 6.4 wt.% of ethanol in fresh matter (equivalent to 169 g/kg DM) was produced. When the 46 wt.% moisture was gathered as vapor from the fermented product, 74% of the produced ethanol was collected. Organic cellular contents (such as the amylase and pronase degradable fractions) were decreased by 63% and organic cell wall (fiber) content by 7% compared to silage prepared from the same material.
Conclusions: We confirmed that efficient ethanol production is induced in nonsterilized whole rice plants in a laboratory-scale solid-state fermentation system. For practical use of the method, further study is needed to scale-up the fermentation volume, develop an efficient ethanol recovery method, and evaluate the fermentation residue as an actual cattle feed.

Web of Science ® 被引用回数 : 9

リンク情報
DOI
https://doi.org/10.1186/1754-6834-4-46
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22040609
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000297351300001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1186/1754-6834-4-46
  • ISSN : 1754-6834
  • PubMed ID : 22040609
  • Web of Science ID : WOS:000297351300001

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