論文

査読有り
2011年3月

Ethanol production from ensiled rice straw and whole-crop silage by the simultaneous enzymatic saccharification and fermentation process

JOURNAL OF BIOSCIENCE AND BIOENGINEERING
  • Yukiko Shinozaki
  • ,
  • Hiroko K. Kitamoto

111
3
開始ページ
320
終了ページ
325
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jbiosc.2010.11.003
出版者・発行元
SOC BIOSCIENCE BIOENGINEERING JAPAN

Silage production from rice straw and whole-plant forage paddy rice is increasing in Japan because of decrease in rice consumption. One potential use for this silage is bioethanol production. In this study, we analyzed the effectiveness of three different commercially available cellulases at saccharification of sun-dried rice straw, ensiled rice straw, and rice whole-crop silage (WCS). Furthermore, the ethanol productivity of the simultaneous saccharification and ethanol fermentation process (SSF) from the same plant substrates was analyzed. Among the three kinds of cellulases tested (Novozymes NS50013, Genencor GC220, and Acremonium cellulase), Acremonium cellulase showed the highest ethanol production for the three plant substrates, and the WCS produced the highest ethanol level. Analysis of the enzymatic degradation activity of the cellulases revealed that Acremonium cellulase contained remarkably high glucoamylase and pectinase side activities relative to the other cellulase preparations. The addition of glucoamylase and pectinase to the other two cellulases significantly increased ethanol productivity to levels observed for the Acremonium cellulase preparation, which showed little enhanced performance with the addition of the same enzymes. Finally, we tested whether milling and sterilization had an effect on ethanol production and found that sterilized silage produced higher ethanol levels but that the milling process had no significant effect. These results show that (i) silage made from whole-plant rice can be used for bioethanol production and (ii) the proper selection and combination of commercially available enzymes can make SSF more cost efficient by removing the need for a pre-treatment step. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.

Web of Science ® 被引用回数 : 30

リンク情報
DOI
https://doi.org/10.1016/j.jbiosc.2010.11.003
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21163697
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000289326600013&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.jbiosc.2010.11.003
  • ISSN : 1389-1723
  • PubMed ID : 21163697
  • Web of Science ID : WOS:000289326600013

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