論文

査読有り
2015年11月

A UV-induced mutant of Cryptococcus flavus GB-1 with increased production of a biodegradable plastic-degrading enzyme

PROCESS BIOCHEMISTRY
  • Takashi Watanabe
  • ,
  • Ken Suzuki
  • ,
  • Yukiko Shinozaki
  • ,
  • Tohru Yarimizu
  • ,
  • Shigenobu Yoshida
  • ,
  • Yuka Sameshima-Yamashita
  • ,
  • Motoo Koitabashi
  • ,
  • Hiroko K. Kitamoto

50
11
開始ページ
1718
終了ページ
1724
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.procbio.2015.07.005
出版者・発行元
ELSEVIER SCI LTD

The yeast strain, Cryptococcus flavus GB-1, isolated from rice husks as a candidate producer of a biodegradable plastic (BP)-degrading enzyme. The genomic DNA sequence encoding the BP-degrading enzyme of GB-1 was determined. The enzyme was named CfCLE because the deduced amino acid sequence (239 amino acids) was found to be 93% identical to that of CLE of Cryptococcus sp. S-2 (accession No. BAK82405.1). The strain GB-1 forms a clear zone (halo) around its colonies on emulsified polybutylene succinate-co-adipate (PBSA) agar plates containing soybean oil but not on plates containing glucose. A UV-induced mutant from this strain, designated as GB-1-DMC1, formed halos on emulsified PBSA plates containing glucose and showed 1.53 U/ml of PBSA-degrading activity in liquid culture with glucose, which was more than 2.5-fold higher than that of the parent strain GB-1 (0.58 U/ml). Moreover, using xylose as a carbon source enhanced the PBSA-degrading activity of both strains. Xylose fed-batch cultivation of GB-1 and GB-1-DMC1 using a 5-1 jar fermentor for 72 h produced 12.1 and 21.2 U/ml of CfCLE, respectively. Purified CfCLE has a molecular mass of 22 kDa, and its optimal pH and temperature for enzyme activity is 7.8 and 45 degrees C, respectively. CfCLE degraded various BP cast films. (C) 2015 Elsevier Ltd. All rights reserved.

Web of Science ® 被引用回数 : 13

リンク情報
DOI
https://doi.org/10.1016/j.procbio.2015.07.005
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000365064800006&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.procbio.2015.07.005
  • ISSN : 1359-5113
  • eISSN : 1873-3298
  • Web of Science ID : WOS:000365064800006

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