論文

査読有り 筆頭著者
2012年7月

Position-dependent FUS-RNA interactions regulate alternative splicing events and transcriptions

SCIENTIFIC REPORTS
  • Shinsuke Ishigaki
  • ,
  • Akio Masuda
  • ,
  • Yusuke Fujioka
  • ,
  • Yohei Iguchi
  • ,
  • Masahisa Katsuno
  • ,
  • Akihide Shibata
  • ,
  • Fumihiko Urano
  • ,
  • Gen Sobue
  • ,
  • Kinji Ohno

2
開始ページ
529
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep00529
出版者・発行元
NATURE PUBLISHING GROUP

FUS is an RNA-binding protein that regulates transcription, alternative splicing, and mRNA transport. Aberrations of FUS are causally associated with familial and sporadic ALS/FTLD. We analyzed FUS-mediated transcriptions and alternative splicing events in mouse primary cortical neurons using exon arrays. We also characterized FUS-binding RNA sites in the mouse cerebrum with HITS-CLIP. We found that FUS-binding sites tend to form stable secondary structures. Analysis of position-dependence of FUS-binding sites disclosed scattered binding of FUS to and around the alternatively spliced exons including those associated with neurodegeneration such as Mapt, Camk2a, and Fmr1. We also found that FUS is often bound to the antisense RNA strand at the promoter regions. Global analysis of these FUS-tags and the expression profiles disclosed that binding of FUS to the promoter antisense strand downregulates transcriptions of the coding strand. Our analysis revealed that FUS regulates alternative splicing events and transcriptions in a position-dependent manner.

リンク情報
DOI
https://doi.org/10.1038/srep00529
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201502865702136288
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22829983
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000306710300004&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/srep00529
  • ISSN : 2045-2322
  • J-Global ID : 201502865702136288
  • PubMed ID : 22829983
  • Web of Science ID : WOS:000306710300004

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