2012年7月
Position-dependent FUS-RNA interactions regulate alternative splicing events and transcriptions
SCIENTIFIC REPORTS
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- 巻
- 2
- 号
- 開始ページ
- 529
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/srep00529
- 出版者・発行元
- NATURE PUBLISHING GROUP
FUS is an RNA-binding protein that regulates transcription, alternative splicing, and mRNA transport. Aberrations of FUS are causally associated with familial and sporadic ALS/FTLD. We analyzed FUS-mediated transcriptions and alternative splicing events in mouse primary cortical neurons using exon arrays. We also characterized FUS-binding RNA sites in the mouse cerebrum with HITS-CLIP. We found that FUS-binding sites tend to form stable secondary structures. Analysis of position-dependence of FUS-binding sites disclosed scattered binding of FUS to and around the alternatively spliced exons including those associated with neurodegeneration such as Mapt, Camk2a, and Fmr1. We also found that FUS is often bound to the antisense RNA strand at the promoter regions. Global analysis of these FUS-tags and the expression profiles disclosed that binding of FUS to the promoter antisense strand downregulates transcriptions of the coding strand. Our analysis revealed that FUS regulates alternative splicing events and transcriptions in a position-dependent manner.
- リンク情報
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- DOI
- https://doi.org/10.1038/srep00529
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201502865702136288
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/22829983
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000306710300004&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1038/srep00529
- ISSN : 2045-2322
- J-Global ID : 201502865702136288
- PubMed ID : 22829983
- Web of Science ID : WOS:000306710300004