論文

査読有り 筆頭著者 国際誌
2020年1月

Characteristic features of porcine endogenous retroviruses in Vietnamese native pigs.

Animal science journal = Nihon chikusan Gakkaiho
  • Shinya Ishihara
  • ,
  • Thanh Q Dang-Nguyen
  • ,
  • Kazuhiro Kikuchi
  • ,
  • Aisaku Arakawa
  • ,
  • Satoshi Mikawa
  • ,
  • Makoto Osaki
  • ,
  • Takeshige Otoi
  • ,
  • Quang Minh Luu
  • ,
  • Thanh Son Nguyen
  • ,
  • Masaaki Taniguchi

91
1
開始ページ
e13336
終了ページ
記述言語
英語
掲載種別
DOI
10.1111/asj.13336

We aimed to clarify the genomic characteristics of porcine endogenous retroviruses (PERVs) in Vietnamese native pig (VnP) breeds. First, we investigated genetic polymorphisms in β- and γ-like PERVs, and we then measured the copy numbers of infectious γ-like PERVs (PERV-A, B, and C). We purified genomic DNA from 15 VnP breeds from 12 regions all over the country and three Western pig breeds as controls, and investigated genetic polymorphisms in all known PERVs, including the beta (β)1-4 and gamma (γ)1-5 groups. PERVs of β1, β2, β3, and γ4 were highly polymorphic with VnP-specific haplotypes. We did not identify genetic polymorphisms in β4, γ1, or γ2 PERVs. We then applied a real-time polymerase chain reaction-based method to estimate copy numbers of the gag, pol, and env genes of γ1 PERVs (defined as A, B, and C). VnP breeds showed significantly lower copy number of the PERV genes compared with the Western pig breeds (on average, 16.2 and 35.7 copies, respectively, p < .05). Two VnP breeds showed significantly higher copy number compared with the other VnPs (p < .05). Our results elucidated that VnPs have specific haplotypes and a low copy number of PERV genes.

リンク情報
DOI
https://doi.org/10.1111/asj.13336
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32219916
ID情報
  • DOI : 10.1111/asj.13336
  • PubMed ID : 32219916

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