2005年3月
Unique characteristics of HIV-1 Vif expression
MICROBES AND INFECTION
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- 巻
- 7
- 号
- 3
- 開始ページ
- 385
- 終了ページ
- 390
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.micinf.2004.11.010
- 出版者・発行元
- ELSEVIER SCIENCE BV
We examined the steady-state expression in cells of four accessory proteins of human immunodeficiency virus type 1 (HIV-1). For this purpose, a series of single gene expression vectors for these viral proteins were constructed and were monitored for their production by transfection. Among them, the expression level of Vif was found to be lowest in both the absence and presence of APOBEC3G. In addition, we noticed the presence of its truncated form, which was not observed for the other accessory proteins. When a subgenomic vector was used for transfection, authentic and several small forms of Vif were produced. By mutational analysis, these forms were demonstrated to be mutant Vif proteins translated from M-8, M-16 and M-29. When a full-length molecular clone was used, the smaller versions of Vif were hardly observed. Functional analysis of these mutant Vif proteins showed that they are incapable of modulating viral infectivity. The results described above, i.e. the low steady-state expression and the presence of truncated forms, represent the unique characteristics of HIV-1 Vif. © 2005 Elsevier SAS. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.micinf.2004.11.010
- ISSN : 1286-4579
- PubMed ID : 15784183
- Web of Science ID : WOS:000229048100007