論文

国際誌
2015年

Pleiotropic Effects of Levofloxacin, Fluoroquinolone Antibiotics, against Influenza Virus-Induced Lung Injury.

PloS one
  • Yuki Enoki
  • ,
  • Yu Ishima
  • ,
  • Ryota Tanaka
  • ,
  • Keizo Sato
  • ,
  • Kazuhiko Kimachi
  • ,
  • Tatsuya Shirai
  • ,
  • Hiroshi Watanabe
  • ,
  • Victor T G Chuang
  • ,
  • Yukio Fujiwara
  • ,
  • Motohiro Takeya
  • ,
  • Masaki Otagiri
  • ,
  • Toru Maruyama

10
6
開始ページ
e0130248
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0130248

Reactive oxygen species (ROS) and nitric oxide (NO) are major pathogenic molecules produced during viral lung infections, including influenza. While fluoroquinolones are widely used as antimicrobial agents for treating a variety of bacterial infections, including secondary infections associated with the influenza virus, it has been reported that they also function as anti-oxidants against ROS and as a NO regulator. Therefore, we hypothesized that levofloxacin (LVFX), one of the most frequently used fluoroquinolone derivatives, may attenuate pulmonary injuries associated with influenza virus infections by inhibiting the production of ROS species such as hydroxyl radicals and neutrophil-derived NO that is produced during an influenza viral infection. The therapeutic impact of LVFX was examined in a PR8 (H1N1) influenza virus-induced lung injury mouse model. ESR spin-trapping experiments indicated that LVFX showed scavenging activity against neutrophil-derived hydroxyl radicals. LVFX markedly improved the survival rate of mice that were infected with the influenza virus in a dose-dependent manner. In addition, the LVFX treatment resulted in a dose-dependent decrease in the level of 8-hydroxy-2'-deoxyguanosine (a marker of oxidative stress) and nitrotyrosine (a nitrative marker) in the lungs of virus-infected mice, and the nitrite/nitrate ratio (NO metabolites) and IFN-γ in BALF. These results indicate that LVFX may be of substantial benefit in the treatment of various acute inflammatory disorders such as influenza virus-induced pneumonia, by inhibiting inflammatory cell responses and suppressing the overproduction of NO in the lungs.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0130248
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26086073
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4473075
ID情報
  • DOI : 10.1371/journal.pone.0130248
  • PubMed ID : 26086073
  • PubMed Central 記事ID : PMC4473075

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