論文

国際誌
2013年1月

Enhanced expression of LAG-3 on lymphocyte subpopulations from persistently lymphocytotic cattle infected with bovine leukemia virus.

Comparative immunology, microbiology and infectious diseases
  • Satoru Konnai
  • ,
  • Saori Suzuki
  • ,
  • Tatsuya Shirai
  • ,
  • Ryoyo Ikebuchi
  • ,
  • Tomohiro Okagawa
  • ,
  • Yuji Sunden
  • ,
  • Claro N Mingala
  • ,
  • Misao Onuma
  • ,
  • Shiro Murata
  • ,
  • Kazuhiko Ohashi

36
1
開始ページ
63
終了ページ
9
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.cimid.2012.09.005

An immunoinhibitory receptor, lymphocyte activation gene-3 (LAG-3), which is mainly expressed in T-cells, is involved in the immune evasion of several pathogens causing chronic infections and tumors. However, unlike human or mouse LAG-3, no functional analysis of LAG-3 has been reported in domestic animals. Thus, in this study, bovine LAG-3 expression was analyzed in bovine leukemia virus (BLV)-infected cattle. In persistent lymphocytotic (PL) cattle, the numbers of LAG-3(+)CD4(+) cells and LAG-3(+)CD8(+) cells were conserved whilst the number of MHC class II(+) cells was remarkably higher than in the control animals. In contrast, the mean fluorescence intensity (MFI) for LAG-3 on PBMCs from PL cattle was significantly increased compared to control and asymptomatic (AL) cattle. Specifically, the LAG-3 expression level was significantly increased in both CD4(+) and CD8(+) T cells from PL cattle. LAG-3 expression correlated positively with increased numbers of lymphocytes and MHC class II(+) cells in infected animals. Preliminary results from PD-L1 and LAG-3 blockade assay revealed that IFN-γ and IL-2 expressions were significantly up-regulated by addition of anti- PD-L1 and LAG-3 antibodies in PBMCs from PL cattle. These findings suggest that LAG-3 might be involved in the inhibition of T-cell function through its binding and signaling on MHC class II molecule during BLV infection.

リンク情報
DOI
https://doi.org/10.1016/j.cimid.2012.09.005
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23146685
ID情報
  • DOI : 10.1016/j.cimid.2012.09.005
  • PubMed ID : 23146685

エクスポート
BibTeX RIS