2010年9月
Aromatic C-H bond hydroxylation by P450 peroxygenases: a facile colorimetric assay for monooxygenation activities of enzymes based on Russig's blue formation
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
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- 巻
- 15
- 号
- 7
- 開始ページ
- 1109
- 終了ページ
- 1115
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s00775-010-0671-9
- 出版者・発行元
- SPRINGER
Aromatic C-H bond hydroxylation of 1-methoxynaphthalene was efficiently catalyzed by the substrate misrecognition system of the hydrogen peroxide dependent cytochrome P450(BS beta) (CYP152A1), which usually catalyzes hydroxylation of long-alkyl-chain fatty acids. Very importantly, the hydroxylation of 1-methoxynaphthalene can be monitored by a color change since the formation of 4-methoxy-1-naphthol was immediately followed by its further oxidation to yield Russig's blue. Russig's blue formation allows us to estimate the peroxygenation activity of enzymes without the use of high performance liquid chromatography, gas chromatography, and nuclear magnetic resonance measurements.
- リンク情報
- ID情報
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- DOI : 10.1007/s00775-010-0671-9
- ISSN : 0949-8257
- PubMed ID : 20490877
- Web of Science ID : WOS:000281597900012