論文

査読有り
2010年9月

Aromatic C-H bond hydroxylation by P450 peroxygenases: a facile colorimetric assay for monooxygenation activities of enzymes based on Russig's blue formation

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
  • Osami Shoji
  • ,
  • Christian Wiese
  • ,
  • Takashi Fujishiro
  • ,
  • Chikako Shirataki
  • ,
  • Bernhard Wuensch
  • ,
  • Yoshihito Watanabe

15
7
開始ページ
1109
終了ページ
1115
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00775-010-0671-9
出版者・発行元
SPRINGER

Aromatic C-H bond hydroxylation of 1-methoxynaphthalene was efficiently catalyzed by the substrate misrecognition system of the hydrogen peroxide dependent cytochrome P450(BS beta) (CYP152A1), which usually catalyzes hydroxylation of long-alkyl-chain fatty acids. Very importantly, the hydroxylation of 1-methoxynaphthalene can be monitored by a color change since the formation of 4-methoxy-1-naphthol was immediately followed by its further oxidation to yield Russig's blue. Russig's blue formation allows us to estimate the peroxygenation activity of enzymes without the use of high performance liquid chromatography, gas chromatography, and nuclear magnetic resonance measurements.

リンク情報
DOI
https://doi.org/10.1007/s00775-010-0671-9
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20490877
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000281597900012&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00775-010-0671-9
  • ISSN : 0949-8257
  • PubMed ID : 20490877
  • Web of Science ID : WOS:000281597900012

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