論文

査読有り
2008年8月

Comparison between polyethylene glycol- and polyethylenimine-mediated transformation of Aspergillus nidulans

CURRENT GENETICS
  • Tetsuya Kuwano
  • ,
  • Chikako Shirataki
  • ,
  • Yasuo Itoh

54
2
開始ページ
95
終了ページ
103
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00294-008-0204-z
出版者・発行元
SPRINGER

Genetic transformation of many filamentous fungi is carried out by a protocol that utilizes polyethylene glycol (PEG) and calcium ion (Ca(2+)). This method has remained practically unchanged for more than 20 years, but the roles these molecules play are not definitively understood. To gain a better understanding, we have compared PEG transformation to a protocol using polyethylenimine (PEI) that is the basis for non-viral transfection in mammals and which has a well established molecular model for assisting DNA uptake. Protoplasts of Aspergillus nidulans could be transformed in the presence of Ca(2+) with a relatively high ratio of PEI to DNA molecules. By comparing PEI and PEG in terms of interaction with DNA, fungal protoplasts, and response to different transformation conditions, we propose that the role of PEG is most likely to function after transforming DNA is incorporated into protoplasts, rather than the accepted view that it functions outside of the cell. Confirmation that protoplast fusion was not involved in DNA uptake is consistent with this hypothesis.

リンク情報
DOI
https://doi.org/10.1007/s00294-008-0204-z
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18649081
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000258152800004&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00294-008-0204-z
  • ISSN : 0172-8083
  • PubMed ID : 18649081
  • Web of Science ID : WOS:000258152800004

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