論文

査読有り
2012年4月

Efficacy of Microarray Profiling Data Combined with QTL Mapping for the Identification of a QTL Gene Controlling the Initial Growth Rate in Rice

PLANT AND CELL PHYSIOLOGY
  • Kenji Yano
  • ,
  • Tomonori Takashi
  • ,
  • Shiro Nagamatsu
  • ,
  • Mikiko Kojima
  • ,
  • Hitoshi Sakakibara
  • ,
  • Hidemi Kitano
  • ,
  • Makoto Matsuoka
  • ,
  • Koichiro Aya

53
4
開始ページ
729
終了ページ
739
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/pcp/pcs027
出版者・発行元
OXFORD UNIV PRESS

Seedling vigor, which is controlled by many quantitative trait loci (QTLs), is one of several important agronomic traits for direct-seedling rice systems. However, isolating these QTL genes is laborious and expensive. Here, we combined QTL mapping and microarray profiling to identify QTL genes for seedling vigor. By performing QTL mapping using 82 backcross inbred lines (BILs) of the Koshihikari (japonica) and Habataki (indica) cultivars for the rice initial growth, we identified two QTLs, early-stage plant development1/2 (qEPD1 and qEPD2), whose Koshihikari alleles promote plant height and/or leaf sheath length. Phenotypic analysis of the two substituted lines carrying the Habataki qEPD1 or qEPD2 allele revealed that qEPD2 functioned more dominantly for the initial growth of rice. From the microarray experiment, 55 and 45 candidate genes were found in the qEPD1 and qEPD2 genomic regions, which are expressed differentially between each substitution line (SL) and Koshihikari. Gibberellin 20 oxidase-2 (OsGA20ox2), which is identical to Semi Dwarf1 (SD1), was included among the 55 candidate genes of qEPD1, whereas its paralog, OsGA20ox1, was included among the 45 candidate genes of qEPD2. Consistently, introduction of the Koshihikari OsGA20ox1 allele into SLqEPD2 increaseed its plant height and leaf sheath length significantly relative to the introduction of the Habataki OsGA20ox1 allele. Therefore, microarray profiling could be useful for rapidly screening QTL candidate genes. We concluded that OsGA20ox1 and OsGA20ox2 (SD1) function during the initial growth of rice, but OsGA20ox1 plays a dominant role in increasing plant height and leaf sheath length at the initial growth stage.

Web of Science ® 被引用回数 : 25

リンク情報
DOI
https://doi.org/10.1093/pcp/pcs027
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22419825
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000302809300013&DestApp=WOS_CPL

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