2007年4月4日
Junctophilin-mediated channel crosstalk essential for cerebellar synaptic plasticity.
The EMBO journal
- 巻
- 26
- 号
- 7
- 開始ページ
- 1924
- 終了ページ
- 33
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/sj.emboj.7601639
- 出版者・発行元
- NATURE PUBLISHING GROUP
Functional crosstalk between cell-surface and intracellular ion channels plays important roles in excitable cells and is structurally supported by junctophilins (JPs) in muscle cells. Here, we report a novel form of channel crosstalk in cerebellar Purkinje cells (PCs). The generation of slow afterhyperpolarization (sAHP) following complex spikes in PCs required ryanodine receptor (RyR)-mediated Ca(2+)-induced Ca(2+) release and the subsequent opening of small-conductance Ca(2+)-activated K(+) (SK) channels in somatodendritic regions. Despite the normal expression levels of these channels, sAHP was abolished in PCs from mutant mice lacking neural JP subtypes (JP-DKO), and this defect was restored by exogenously expressing JPs or enhancing SK channel activation. The stimulation paradigm for inducing long-term depression (LTD) at parallel fiber-PC synapses adversely established long-term potentiation in the JP-DKO cerebellum, primarily due to the sAHP deficiency. Furthermore, JP-DKO mice exhibited impairments of motor coordination and learning, although normal cerebellar histology was retained. Therefore, JPs support the Ca(2+)-mediated communication between voltage-gated Ca(2+) channels, RyRs and SK channels, which modulates the excitability of PCs and is fundamental to cerebellar LTD and motor functions.
- リンク情報
-
- DOI
- https://doi.org/10.1038/sj.emboj.7601639
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/17347645
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1847665
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000245465800016&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1038/sj.emboj.7601639
- ISSN : 0261-4189
- PubMed ID : 17347645
- PubMed Central 記事ID : PMC1847665
- Web of Science ID : WOS:000245465800016