2014年1月1日
Centrifuge-based microfluidic device for trapping single cells in a drop
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
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- 開始ページ
- 1410
- 終了ページ
- 1412
© 14CBMS. We describe a centrifuge-based dynamic microarray system for trapping an array of single cells from small amount of suspension. We optimized microchannel designs and centrifugal force for minimizing sample volumes and maximizing trapping efficiencies, and achieved single-cell trapping by introducing only 5 μl of cell suspension with the centrifugal force of 70 G. Trapping of cells stained with DAPI shows that single cells were trapped at desired trapping spots. This system will be applicable for single cell assays using rare or expensive cells.
- ID情報
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- SCOPUS ID : 84941696035