論文

査読有り 国際誌
2017年12月

Interneurons secrete prosaposin, a neurotrophic factor, to attenuate kainic acid-induced neurotoxicity.

IBRO reports
  • Hiroaki Nabeka
  • Shouichiro Saito
  • Xuan Li
  • Tetsuya Shimokawa
  • Md Sakirul Islam Khan
  • Kimiko Yamamiya
  • Soichiro Kawabe
  • Takuya Doihara
  • Fumihiko Hamada
  • Naoto Kobayashi
  • Seiji Matsuda
  • 全て表示

3
開始ページ
17
終了ページ
32
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.ibror.2017.07.001

Prosaposin (PS) is a secretory neurotrophic factor, as well as a regulator of lysosomal enzymes. We previously reported the up-regulation of PS and the possibility of its axonal transport by GABAergic interneurons after exocitotoxicity induced by kainic acid (KA), a glutamate analog. In the present study, we performed double immunostaining with PS and three calcium binding protein markers: parvalbumin (PV), calbindin, and calretinin, for the subpopulation of GABAergic interneurons, and clarified that the increased PS around the hippocampal pyramidal neurons after KA injection existed mainly in the axons of PV positive interneurons. Electron microscopy revealed PS containing vesicles in the PV positive axon. Double immunostaining with PS and secretogranin or synapsin suggested that PS is secreted with secretogranin from synapses. Based on the results from in situ hybridization with two alternative splicing forms of PS mRNA, the increase of PS in the interneurons was due to the increase of PS + 0 (mRNA without 9-base insertion) as in the choroid plexus, but not PS + 9 (mRNA with 9-base insertion). These results were similar to those from the choroid plexus, which secretes an intact form PS + 0 to the cerebrospinal fluid. Neurons, especially PV positive GABAergic interneurons, produce and secrete the intact form of PS around hippocampal pyramidal neurons to protect them against KA neurotoxicity.

リンク情報
DOI
https://doi.org/10.1016/j.ibror.2017.07.001
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30135939
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6084830
ID情報
  • DOI : 10.1016/j.ibror.2017.07.001
  • PubMed ID : 30135939
  • PubMed Central 記事ID : PMC6084830

エクスポート
BibTeX RIS