2017年8月
NeuN immunoreactivity in the brain of Xenopus laevis.
Tissue & cell
- ,
- ,
- 巻
- 49
- 号
- 4
- 開始ページ
- 514
- 終了ページ
- 519
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.tice.2017.05.001
- 出版者・発行元
- CHURCHILL LIVINGSTONE
Neuronal nuclear antigen (NeuN), discovered in mice brain cell nuclei by Mullen et al. (1992), is used as an excellent marker of post-mitotic neurons in vertebrates. In this study, the expression pattern of NeuN was examined in the Xenopus brain to explore phylogenetic differences in NeuN expression. Anti-NeuN antibody showed selective staining in mouse and Xenopus brain extracts, but the number and molecular weight of the bands differed in Western blotting analysis. In immunostaining, anti-NeuN antibody showed selective staining of neurons, but not glial cells, in the Xenopus brain. Most neurons, including olfactory bulb mitral cells and cerebellar Purkinjie cells, which show no immunoreactivity in birds/mammals, showed NeuN immunoreactivity in Xenopus. This study revealed that anti-NeuN antibody is a useful marker of post-mitotic neurons in amphibians, but it also stains neurons that show no reactivity in more derived animals.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.tice.2017.05.001
- ISSN : 0040-8166
- PubMed ID : 28558928
- Web of Science ID : WOS:000408454400008