Papers

Peer-reviewed International journal
May 24, 2022

Volumetric Strategy for Quantitatively Elucidating a Local Hydration Network around a G-Quadruplex.

Analytical chemistry
  • Saki Matsumoto
  • ,
  • Shuntaro Takahashi
  • ,
  • Sudipta Bhowmik
  • ,
  • Tatsuya Ohyama
  • ,
  • Naoki Sugimoto

Volume
94
Number
20
First page
7400
Last page
7407
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1021/acs.analchem.2c01075

Hydration around nucleic acids, such as DNA and RNA, is an important factor not only for the stability of nucleic acids but also for their interaction with binding molecules. Thus, it is necessary to quantitatively elucidate the hydration properties of nucleic acids around a certain structure. In this study, volumetric changes in G-quadruplex (G4) RNA formation were investigated by systematically changing the number of G-quartet stacks under high pressure. The volumetric contribution at the level of each G4 structural unit revealed that the core G4 helix was significantly more dehydrated than the other parts, including the edges of G-quartets and loops. These findings will help in predicting the binding of G4 ligands on the surface of G4, depending on the chemical structure of the ligand and solution environment. Therefore, the preset volumetric parameter provides information that can predict molecular interactions in G4 formations during molecular crowding in cells.

Link information
DOI
https://doi.org/10.1021/acs.analchem.2c01075
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/35535999
ID information
  • DOI : 10.1021/acs.analchem.2c01075
  • Pubmed ID : 35535999

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