May 24, 2022
Volumetric Strategy for Quantitatively Elucidating a Local Hydration Network around a G-Quadruplex.
Analytical chemistry
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- Volume
- 94
- Number
- 20
- First page
- 7400
- Last page
- 7407
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1021/acs.analchem.2c01075
Hydration around nucleic acids, such as DNA and RNA, is an important factor not only for the stability of nucleic acids but also for their interaction with binding molecules. Thus, it is necessary to quantitatively elucidate the hydration properties of nucleic acids around a certain structure. In this study, volumetric changes in G-quadruplex (G4) RNA formation were investigated by systematically changing the number of G-quartet stacks under high pressure. The volumetric contribution at the level of each G4 structural unit revealed that the core G4 helix was significantly more dehydrated than the other parts, including the edges of G-quartets and loops. These findings will help in predicting the binding of G4 ligands on the surface of G4, depending on the chemical structure of the ligand and solution environment. Therefore, the preset volumetric parameter provides information that can predict molecular interactions in G4 formations during molecular crowding in cells.
- Link information
- ID information
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- DOI : 10.1021/acs.analchem.2c01075
- Pubmed ID : 35535999