ISHIKAWA Shu

J-GLOBAL         Last updated: Sep 14, 2019 at 02:50
 
Avatar
Name
ISHIKAWA Shu
Affiliation
Kobe University
Section
Graduate School of Science, Technology and Innovation Department of Science, Technology and Innovation
Job title
Associate Professor

Research Areas

 
 

Academic & Professional Experience

 
Apr 2016
 - 
Today
Associate Professor, Graduate School of Science, Technology and Innovation, Kobe University
 
Nov 2001
 - 
Mar 2016
Assistant professor, Nara Institute of Science and Technology
 
Apr 2001
 - 
Oct 2001
Research Fellowship, Japan Society for the Promotion of Science (JSPS)
 

Education

 
Apr 1996
 - 
Mar 1999
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
 

Published Papers

 
Nishihata Shogo, Kondo Takahiko, Tanaka Kosei, Ishikawa Shu, Takenaka Shinji, Kang Choong-Min, Yoshida Ken-ichi
BMC Microbiology   18    Oct 2018   [Refereed]
Background Bradyrhizobium diazoefficiens USDA110 nodulates soybeans for nitrogen fixation. It accumulates poly-3-hydroxybutyrate (PHB), which is of physiological importance as a carbon/energy source for survival during starvation, infection, and n...
Miyano Megumi, Tanaka Kosei, Ishikawa Shu, Mori Kotaro, Miguel-Arribas Andres, Meijer Wilfried J. J., Yoshida Ken-ichi
Microbial Cell Factories   17    Aug 2018   [Refereed]
Background: Bacterial strains of the genus Geobacillus grow at high temperatures of 50-75 °C and could thus be useful for biotechnological applications. However, genetic manipulation of these species is difficult because the current techniques for...
Miyano Megumi, Tanaka Kosei, Ishikawa Shu, Takenaka Shinji, Miguel-Arribas Andres, Meijer Wilfried J. J., Yoshida Ken-ichi
MICROBIAL CELL FACTORIES   17    Jan 2018   [Refereed]
Miyano Megumi, Tanaka Kosei, Ishikawa Shu, Takenaka Shinji, Miguel-Arribas Andres, Meijer Wilfried J. J., Yoshida Ken-ichi
Microbial Cell Factories   17    Jan 2018   [Refereed]
Background: The conjugative plasmid, pLS20, isolated from Bacillus subtilis natto, has an outstanding capacity for rapid self-transfer. In addition, it can function as a helper plasmid, mediating the mobilization of an independently replicating co...
Kang Dong-Min, Michon Christophe, Morinaga Tetsuro, Tanaka Kosei, Takenaka Shinji, Ishikawa Shu, Yoshida Ken-ichi
BMC MICROBIOLOGY   17    Jul 2017   [Refereed]
Tanaka Kosei, Natsume Ayane, Ishikawa Shu, Takenaka Shinji, Yoshida Ken-ichi
Microbial Cell Factories   16    2017   [Refereed]
Kang Dong-Min, Tanaka Kosei, Takenaka Shinji, Ishikawa Shu, Yoshida Ken-ichi
Bioscience Biotechnology and Biochemistry   81(5) 1026-1032   2017   [Refereed]
Chumsakul Onuma, Anantsri Divya P, Quirke Tai, Oshima Taku, Nakamura Kensuke, Ishikawa Shu, Nakano Michiko M.
Journal of Bacteriology   199(13)    2017   [Refereed]
Terakawa Ayako, Natsume Ayane, Okada Atsushi, Nishihata Shogo, Kuse Junko, Tanaka Kosei, Takenaka Shinji, Ishikawa Shu, Yoshida Ken-ichi
Bmc Microbiology   16(1) 1-13   2016   [Refereed]
Higashi Koichi, Tobe Toru, Kanai Akinori, Uyar Ebru, Ishikawa Shu, Suzuki Yutaka, Ogasawara Naotake, Kurokawa Ken, Oshima Taku
Plos Genetics   12(1)    2016   [Refereed]
Akiyama Masahiro Tatsumi, Oshima Taku, Chumsakul Onuma, Ishikawa Shu, Maki Hisaji
Genes To Cells   21(8) 914   2016   [Refereed]
TOYA YOSHIHIRO, TOYA YOSHIHIRO, HIRASAWA TAKASHI, HIRASAWA TAKASHI, HIRASAWA TAKASHI, ISHIKAWA SHU, ISHIKAWA SHU, CHUMSAKUL ONUMA, CHUMSAKUL ONUMA, MORIMOTO TAKUYA, MORIMOTO TAKUYA
Biosci Biotechnol Biochem   79(12) 2073-2080   Dec 2015   [Refereed]
MALIK AMARILA, HAPSARI MARIA TYAS, OHTSU IWAO, ISHIKAWA SHU, TAKAGI HIROSHI
J Biosci Bioeng   119(5) 515-520   May 2015   [Refereed]
Malik Amarila, Hapsari Maria Tyas, Ohtsu Iwao, Ishikawa Shu, Takagi Hiroshi
Journal of Bioscience and Bioengineering   119(5) 515-520   2015   [Refereed]
Toya Yoshihiro, Hirasawa Takashi, Ishikawa Shu, Chumsakul Onuma, Morimoto Takuya, Liu Shenghao, Masuda Kenta, Kageyama Yasushi, Ozaki Katsuya, Ogasawara Naotake, Shimizu Hiroshi
Bioscience Biotechnology and Biochemistry   79(12) 2073-2080   2015   [Refereed]
Tanaka Kosei, Iwasaki Kana, Morimoto Takuya, Matsuse Takatsugu, Hasunuma Tomohisa, Takenaka Shinji, Chumsakul Onuma, Ishikawa Shu, Ogasawara Naotake, Yoshida Ken-ichi
Bmc Microbiology   15(1)    2015   [Refereed]
Murayama Satohiko, Ishikawa Shu, Chumsakul Onuma, Ogasawara Naotake, Oshima Taku
Plos One   10(7)    2015   [Refereed]
HENARES BERNADETTE, KOMMINENI SUSHMA, CHUMSAKUL ONUMA, OGASAWARA NAOTAKE, ISHIKAWA SHU, NAKANO MICHIKO M.
J Bacteriol   196(2) 493-503   Jan 2014   [Refereed]
Henares Bernadette, Kommineni Sushma, Chumsakul Onuma, Ogasawara Naotake, Ishikawa Shu, Nakano Michiko M.
Journal of Bacteriology   196(2) 493-503   2014   [Refereed]
LEI YING, OSHIMA TAKU, OGASAWARA NAOTAKE, ISHIKAWA SHU
J Bacteriol   195(8) 1697-1705   Apr 2013   [Refereed]
Lei Ying, Oshima Taku, Ogasawara Naotake, Ishikawa Shu
Journal of Bacteriology   195(8) 1697-1705   2013   [Refereed]
Ueda Takeshi, Takahashi Hiroki, Uyar Ebru, Ishikawa Shu, Ogasawara Naotake, Oshima Taku
DNA Research   20(3) 263-271   2013   [Refereed]
Chumsakul Onuma, Nakamura Kensuke, Kurata Tetsuya, Sakamoto Tomoaki, Hobman Jon L, Ogasawara Naotake, Oshima Taku, Ishikawa Shu
DNA Research   20(4) 325-337   2013   [Refereed]
Duman Ramona, Ishikawa Shu, Celik Ilkay, Strahl Henrik, Ogasawara Naotake, Troc Paulina, Loewe Jan, Hamoen Leendert W.
Proceedings of the National Academy of Sciences of the United States of America   110(48) 4601-4610   2013   [Refereed]
大島拓, 石川周
化学と生物   51(10) 670-678   2013   [Refereed]
2Ap03 Bacillus subtilis cell factory for production of scyllo-inositol promising for Alzheimer's disease (Bio-Based Production) :
YOSHIDA Ken-ichi, ONUMA Chumsakul, ISHIKAWA Shu, OGASAWARA Naotake
日本生物工学会大会講演要旨集   64    2012
Ishikawa Shu, Okumura Hajime, Yoshimura Mika, Ueki Mikako, Oshima Taku, Ogasawara Naotake
Genes & Genetic Systems   87(6) 385   2012   [Refereed]
OKUMURA HAJIME, YOSHIMURA MIKA, UEKI MIKAKO, OSHIMA TAKU, OGASAWARA NAOTAKE, ISHIKAWA SHU
Nucleic Acids Res   40(1) 220-234   2012   [Refereed]
Okumura Hajime, Yoshimura Mika, Ueki Mikako, Oshima Taku, Ogasawara Naotake, Ishikawa Shu
Nucleic Acids Research   40(1) 220-234   2012   [Refereed]
KUSUYA YOKO, KUROKAWA KEN, ISHIKAWA SHU, OGASAWARA NAOTAKE, OSHIMA TAKU
J Bacteriol   193(12) 3090-3099   Jun 2011   [Refereed]
Kawai Yoshikazu, Marles-Wright Jon, Cleverley Robert M, Emmins Robyn, Ishikawa Shu, Kuwano Masayoshi, Heinz Nadja, Nhat Khai Bui, Hoyland Christopher N, Ogasawara Naotake, Lewis Richard J, Vollmer Waldemar, Daniel Richard A, Errington Jeff
Embo Journal   30(24) 4931-4941   2011   [Refereed]
CHUMSAKUL ONUMA, TAKAHASHI HIROKI, OSHIMA TAKU, HISHIMOTO TAKAHIRO, KANAYA SHIGEHIKO, OGASAWARA NAOTAKE, ISHIKAWA SHU
Nucleic Acids Res   39(2) 414-428   2011   [Refereed]
Chumsakul Onuma, Takahashi Hiroki, Oshima Taku, Hishimoto Takahiro, Kanaya Shigehiko, Ogasawara Naotake, Ishikawa Shu
Nucleic Acids Research   39(2) 414-428   2011   [Refereed]
Malik Amarila, Ishikawa Shu, Sahlan Muhamad, Ogasawara Naotake, Uyen Quynh Nguyen, Suryadi Herman
African Journal of Biotechnology   10(74) 16915-16923   2011   [Refereed]
Nakamura Kensuke, Oshima Taku, Morimoto Takuya, Ikeda Shun, Yoshikawa Hirofumi, Shiwa Yuh, Ishikawa Shu, Linak Margaret C, Hirai Aki, Takahashi Hiroki, Altaf-Ul-Amin Md, Ogasawara Naotake, Kanaya Shigehiko
Nucleic Acids Research   39(13)    2011   [Refereed]
Kusuya Yoko, Kurokawa Ken, Ishikawa Shu, Ogasawara Naotake, Oshima Taku
Journal of Bacteriology   193(12) 3090-3099   2011   [Refereed]
ISHIKAWA SHU, OSHIMA TAKU, KUROKAWA KEN, KUSUYA YOKO, OGASAWARA NAOTAKE
J Bacteriol   192(21) 5778-5787   Nov 2010   [Refereed]
Ishikawa Shu, Oshima Taku, Kurokawa Ken, Kusuya Yoko, Ogasawara Naotake
Journal of Bacteriology   192(21) 5778-5787   2010   [Refereed]
Uyar Ebru, Kurokawa Ken, Yoshimura Mika, Ishikawa Shu, Ogasawara Naotake, Oshima Taku
Journal of Bacteriology   191(7) 2388-2391   2009   [Refereed]
Wu Ling Juan, Ishikawa Shu, Kawai Yoshikazu, Oshima Taku, Ogasawara Naotake, Errington Jeff
Embo Journal   28(13) 1940-1952   2009   [Refereed]
Cho Eunha, Ogasawara Naotake, Ishikawa Shu
Genes & Genetic Systems   83(2) 111-125   2008   [Refereed]
石川周, 小笠原直毅
蛋白質核酸酵素   53(13) 1725-1731   2008   [Refereed]
ISHIKAWA SHU, OGURA YOSHITOSHI, YOSHIMURA MIKA, OKUMURA HAJIME, CHO EUNHA, KAWAI YOSHIKAZU, KUROKAWA KEN, OSHIMA TAKU, OGASAWARA NAOTAKE
DNA Res   14(4) 155-168   Aug 2007   [Refereed]
Ishikawa Shu, Ogura Yoshitoshi, Yoshimura Mika, Okumura Hajime, Cho Eunha, Kawai Yoshikazu, Kurokawa Ken, Oshima Taku, Ogasawara Naotake
DNA Research   14(4) 155-168   2007   [Refereed]
ISHIKAWA SHU, KAWAI YOSHIKAZU, HIRAMATSU KONOSUKE, KUWANO MASAYOSHI, OGASAWARA NAOTAKE
Mol Microbiol   60(6) 1364-1380   Jun 2006   [Refereed]
Ishikawa S, Kawai Y, Hiramatsu K, Kuwano M, Ogasawara N
Molecular Microbiology   60(6) 1364-1380   2006   [Refereed]
Oshima Taku, Ishikawa Shu, Kurokawa Ken, Aiba Hirofumi, Ogasawara Naotake
DNA Research   13(4) 141-153   2006   [Refereed]
BONGIORNI CRISTINA, ISHIKAWA SHU, STEPHENSON SOPHIE, OGASAWARA NAOTAKE, PEREGO MARTA
J Bacteriol   187(13) 4353-4361   Jul 2005   [Refereed]
Bongiorni C, Ishikawa S, Stephenson S, Ogasawara N, Perego M
Journal of Bacteriology   187(13) 4353-4361   2005   [Refereed]
FUKUSHIMA Tatsuya, ISHIKAWA Shu, YAMAMOTO Hiroki, OGASAWARA Naotake, SEKIGUCHI Junichi
Journal of Biochemistry   133(4) 475-483   Apr 2003   [Refereed]
FUKUSHIMA T, ISHIKAWA S, YAMAMOTO H, OGASAWARA N, SEKIGUCHI J
J Biochem   133(4) 475-483   Apr 2003   [Refereed]
Kobayashi K, Ehrlich SD, Albertini A, Amati G, Andersen KK, Arnaud M, Asai K, Ashikaga S, Aymerich S, Bessieres P, Boland F, Brignell SC, Bron S, Bunai K, Chapuis J, Christiansen LC, Danchin A, Debarbouille M, Dervyn E, Deuerling E, Devine K, Devine SK, Dreesen O, Errington J, Fillinger S, Foster SJ, Fujita Y, Galizzi A, Gardan R, Eschevins C, Fukushima T, Haga K, Harwood CR, Hecker M, Hosoya D, Hullo MF, Kakeshita H, Karamata D, Kasahara Y, Kawamura F, Koga K, Koski P, Kuwana R, Imamura D, Ishimaru M, Ishikawa S, Ishio I, Le Coq D, Masson A, Mauel C, Meima R, Mellado RP, Moir A, Moriya S, Nagakawa E, Nanamiya H, Nakai S, Nygaard P, Ogura M, Ohanan T, O'Reilly M, O'Rourke M, Pragai Z, Pooley HM, Rapoport G, Rawlins JP, Rivas LA, Rivolta C, Sadaie A, Sadaie Y, Sarvas M, Sato T, Saxild HH, Scanlan E, Schumann W, Seegers JFML, Sekiguchi J, Sekowska A, Seror SJ, Simon M, Stragier P, Studer R, Takamatsu H, Tanaka T, Takeuchi M, Thomaides HB, Vagner V, van Dijl JM, Watabe K, Wipat A, Yamamoto H, Yamamoto M, Yamamoto Y, Yamane K, Yata K, Yoshida K, Yoshikawa H, Zuber U, Ogasawara N
Proceedings of the National Academy of Sciences of the United States of America   100(8) 4678-4683   2003   [Refereed]
Fukushima T, Ishikawa S, Yamamoto H, Ogasawara N, Sekiguchi J
Journal of Biochemistry   133(4) 475-483   2003   [Refereed]
SHIDA T, MUKAIJO K, ISHIKAWA S, YAMAMOTO H, SEKIGUCHI J
Biosci Biotechnol Biochem   66(7) 1555-1558   Jul 2002   [Refereed]
SHIDA Toshio, MUKAIJO Kohsuke, ISHIKAWA Shu, YAMAMOTO Hiroki, SEKIGUCHI Junichi
Bioscience, biotechnology, and biochemistry   66(7) 1555-1558   Jul 2002   [Refereed]
A hyper extracellular protein producer, Bacillus subtilis 327UH, produced large amounts of levan in a medium containing 20% sucrose, and the yield of levan after 10 hours was more than 60%, when based on the fructose amount of sucrose. After trans...
Ishikawa S, Core L, Perego M
Journal of Biological Chemistry   277(23) 20483-20489   2002   [Refereed]
Shida T, Mukaijo K, Ishikawa S, Yamamoto H, Sekiguchi J
Bioscience Biotechnology and Biochemistry   66(7) 1555-1558   2002   [Refereed]
Core LJ, Ishikawa S, Perego M
Peptides   22(10) 1549-1553   2001   [Refereed]
Peptidoglycan Hydrolase LytF Plays a Role in Cell Separation with CwlF during Vegetative Growth of Bacillus subtilis.
OHNISHI R, ISHIKAWA S, SEKIGUCHI J
J Bacteriol   181(10) 3178-3184   May 1999   [Refereed]
Sekiguchi J, Sato T, Nanamiya H, Ohashi Y, Kawamura F, Takamatsu H, Kodama T, Watabe K, Ishikawa S.
Tanpakushitsu Kakusan Koso   44(10) 1460-6   1999   [Refereed]
Genome analysis and germination related genes of Bacillus subtilis
Fukushima Tatsuya, Ishikawa Shu, Yamamoto Hiroki, Sekiguchi Junichi
日本生物工学会大会講演要旨集   11    1999   [Refereed]
Ishikawa S, Kawahara S, Sekiguchi J
Molecular and General Genetics   262(4/5) 738-748   1999   [Refereed]
Ohnishi R, Ishikawa S, Sekiguchi J
Journal of Bacteriology   181(10) 3178-3184   1999   [Refereed]
Regulation of a New Cell Wall Hydrolase Gene, cwlF, Which Affects Cell Separation in Bacillus subtilis.
ISHIKAWA S, HARA Y, OHNISHI R, SEKIGUCHI J
J Bacteriol   180(9) 2549-2555   May 1998   [Refereed]
Regulation and Characterization of a Newly Deduced Cell Wall Hydrolase Gene (cwlJ) Which Affects Germination of Bacillus subtilis Spores.
ISHIKAWA S, YAMANE K, SEKIGUCHI J
J Bacteriol   180(6) 1375-1380   Mar 1998   [Refereed]
Ishikawa S, Yamane K, Sekiguchi J
Journal of Bacteriology   180(6) 1375-1380   1998   [Refereed]
Ishikawa S, Hara Y, Ohnishi R, Sekiguchi J
Journal of Bacteriology   180(9) 2549-2555   1998   [Refereed]
Localization of proteins onto the cell surface of Bacillus subtilis with a cell wall binding domain of autolysin
Tsuchiya Atsushi, Ishikawa Shu, Sekiguchi Junichi
日本生物工学会大会講演要旨集   10    1998
Study on a cw1J gene which affects germination of Bacillus subtilis spores
Ishikawa Shu, Yamane Kunio, Sekiguchi Junichi
日本生物工学会大会講演要旨集   9    1997
Study on a Bacillus subtilis autolysin, Cw1F, which appears during vegetative growth
Hara Yoshiko, Ohnishi Ryo, Ishikawa Shu, Sekiguchi Junichi
日本生物工学会大会講演要旨集   9    1997
Kawahara S, Utsunomiya C, Ishikawa S, Sekiguchi J
Journal of Fermentation and Bioengineering   83(5) 419-422   1997   [Refereed]

Misc

 
2Ap03 Bacillus subtilis cell factory for production of scyllo-inositol promising for Alzheimer's disease (Bio-Based Production)
YOSHIDA Ken-ichi, ONUMA Chumsakul, ISHIKAWA Shu, OGASAWARA Naotake
日本生物工学会大会講演要旨集   64    Oct 2012
Functional analysis of the Veg protein that stimulates biofilm formation in Bacillus subtilis
LEI YING, ISHIKAWA SHU, OGASAWARA NAOTAKE
日本ゲノム微生物学会年会要旨集   6th 85   2012
Bacillus subtilis degU32(hy) proactively cancels catabolite repression
IWASAKI KANA, ISHIKAWA SHU, OGASAWARA NAOTAKE, TAKENAKA SHINJI, YOSHIDA KEN-ICHI
日本分子生物学会年会プログラム・要旨集(Web)   34th 2P-0003 (WEB ONLY)   2011
Transcription factor GreA contributes to resolve promoter proximal pausing of RNA polymerase in B. subtilis cells.
KUSUYA YOKO, OSHIMA TAKU, ISHIKAWA SHU, KUROKAWA KEN, OGASAWARA NAOTAKE
日本分子生物学会年会プログラム・要旨集(Web)   34th 3P-0215 (WEB ONLY)   2011
Search of nucleoid proteins in Bacillus subtilis: comparative analysis using ChAP(ChIP)-chip analysis between in B. subtilis and E. coli.
CHUMSAKUL ONUMA, UYAL EBRU, ISHIKAWA SHU, OSHIMA TAKU, OGASAWARA NAOTAKE
日本分子生物学会年会講演要旨集   32nd(Vol.1) 27   2009

Books etc

 
GeF-seq: A Simple Procedure for Base Pair Resolution ChIP-seq
Onuma Chumsakul, Kensuke Nakamura, ISHIKAWA SHU, Taku Oshima (Part:Joint Work)
Humana Press, New York, NY   2018   
高精度で結合領域を決定するGeF-seq
大島 拓, 石川 周, Chumsakul Onuma, 中村 建介 (Part:Joint Work)
羊土社   Sep 2014   
細菌の発現制御機構をゲノムワイドに解析する :次世代シーケンサーを用いた高精度な網羅的解析の可能性
大島 拓, 石川 周
日本農芸化学会   Oct 2013   
細菌の細胞分裂の分子メカニズム
石川 周, 小笠原 直毅 (Part:Joint Work)
共立出版   Oct 2008   
枯草菌の胞子形成,胞子,発芽
佐藤 勉, 七宮 英晃, 大橋 由明, 河村 富士夫, 高松 宏治, 児玉 武子, 渡部 一仁, 石川 周, 関口 順一 (Part:Joint Work)
共立出版   Aug 1999   

Teaching Experience

 

Research Grants & Projects

 
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2015 - 2017    Investigator(s): Shu ISHIKAWA
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2012 - 2014    Investigator(s): Shu ISHIKAWA
細菌の細胞分裂はFtsZが中心となり行われるが、FtsZは細胞膜に結合できないので、細胞膜に繋ぎとめる蛋白質が必須である。大腸菌ではFtsAが、シアノバクテリアではSepFがその役割を担うと考えられるが、増殖に必須である。枯草菌は両方の因子を備え、さらに同様の役割をすると考えられるEzrAも有するので、各々が破壊可能である。本研究では、酵母2ハブリッド解析、結晶構造、電子顕微鏡観察など多面的に調べ、SepFがFtsZを膜につなぎとめるメカニズムを解明した。また、EzrAがFtsZと直接結合...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2011 - 2013    Investigator(s): Hiroki YAMAMOTO
枯草菌が桿状形態を維持して増殖するためには、細胞壁溶解酵素の活性が必要である。本研究では、これらの酵素の機能を制御する因子の解明を試みた。まず、LytEのシグナルペプチド(SPLytE)を用いてLytFを発現する株(SPLytE-LytF)では、分泌されたLytFは本来のセプタムと極だけでなく細胞側壁にも局在していた。また、lytEとcwlOの合成致死性についても、部分的ではあるものの相補できるようになっていた。以上の結果から、シグナルペプチドが細胞壁溶解酵素の局在性と機能を支配している可...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(A))
Project Year: 2011 - 2013    Investigator(s): Naotake OGASAWARA
我々は、1bpの解像度でDNA結合タンパク質とゲノムDNAとの結合領域を網羅的に解析可能なGeF-seq法を新たに開発し、進化的に大きく異なる細菌、大腸菌と枯草菌の核様体タンパク質について解析を行った。その結果、配列非特異的な結合様式を示すHUタンパク質は、枯草菌、大腸菌で同様の結合様式を示すものの、大腸菌では、DNAが屈曲あるいはヘアピン状の構造を取りうるDNA配列に、FisおよびIHFタンパク質が結合し、DNAと構造を取ることが示唆された。これらのタンパク質は枯草菌には存在しないことか...
Japan Science and Technology Agency (JST): Advanced Low Carbon Technology (ALCA)
Project Year: 2010 - 2016    Investigator(s): Notake OGASAWARA
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2007 - 2009    Investigator(s): Hiroki YAMAMOTO
レクチンの一種であるConcanavalin Aの蛍光標識物(ConA-TMR)を用いることにより、枯草菌の細胞壁テイコ酸(WTA)を特異的に検出する方法を確立した。種々のテイコ酸修飾遺伝子変異株を用いて観察を行った結果、ConA-TMRはmajor WTAのグルコース修飾を効率よく検出し、minor WTAやリポテイコ酸は検出できないことが明らかになった。また、major WTAのグルコース修飾に関与するtagE条件変異株を用いて、細胞側壁におけるWTA修飾が螺旋状に行われていることを明...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(特定領域研究)
Project Year: 2006 - 2007    Investigator(s): Shu ISHIKAWA
細菌細胞の増殖を支える遺伝子・タンパク質のネットワークがどのように構築されているか、その基本原理の解明を目指して、2大モデル細菌である枯草菌・大腸菌を対象として、タイリングチップを用いたChIP-chip解析や質量分析法を用いた複合来解析等の新たな研究手法の導入を進め、新たな必須遺伝子機能、必須遺伝子産物間の機能ネットワーク、転写調節タンパク質と核様体タンパク質による転写制御ネットワーク、核様体タンパク質による細胞周期制御、代謝ネットワークのモデル化等について、多くの新たな知見を明らかにした。
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(A))
Project Year: 2005 - 2007    Investigator(s): Naotake OGASAWARA
枯草菌は、モデル生物として、ゲノム機能解析が進んでいる。その中で、ゲノム配列決定によって見出された機能未知、あるいは、未解析である遺伝子の破壊株ライブラリーの作成が、研究代表者を中心として行われた(Proc Natl Acad Sci USA,100,4678-4683,2003)。その結果、LB培地・37度という培養条件で、その破壊が致死となる必須遺伝子は271であるということが明らかとなった。その内、機能が不明確なものは22遺伝子であった。興味深いことに、その中の7遺伝子は、分子スイッ...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(若手研究(B))
Project Year: 2005 - 2009    Investigator(s): Naotake OGASAWARA
FtsZは自己縮合してポリマー構造、さらにはリング構造を形成し、細菌の細胞分裂の基盤となる。この様な反応には、枯草菌ではFtsZと直接結合するFtsA、YlmF、ZapAとの結合が重要である。そこで、このような細胞分裂初期の分子メカニズムを解明するために、FtsZにランダムに変異を導入し、相互作用を失う変異FtsZを酵母2ハイブリッド解析により同定し、どのアミノ酸がFtsZ自身やFtsA、YlmF、ZapAとの結合に関わっているかを決定した。FtsZは、Plus End領域、T7 loop...

Patents

 
増田 健太, 劉 生浩, 森本 拓也, 小笠原 直毅, 石川 周, チュムサクル オンウマ
増田 健太, 劉 生浩, 森本 拓也, 小笠原 直毅, 石川 周, チュムサクル オンウマ
増田 健太, 劉 生浩, 森本 拓也, 小笠原 直毅, 石川 周, チュムサクル オンウマ