論文

査読有り 国際誌
2018年6月29日

Growth Cone Phosphoproteomics Reveals that GAP-43 Phosphorylated by JNK Is a Marker of Axon Growth and Regeneration.

iScience
  • Asami Kawasaki
  • ,
  • Masayasu Okada
  • ,
  • Atsushi Tamada
  • ,
  • Shujiro Okuda
  • ,
  • Motohiro Nozumi
  • ,
  • Yasuyuki Ito
  • ,
  • Daiki Kobayashi
  • ,
  • Tokiwa Yamasaki
  • ,
  • Ryo Yokoyama
  • ,
  • Takeshi Shibata
  • ,
  • Hiroshi Nishina
  • ,
  • Yutaka Yoshida
  • ,
  • Yukihiko Fujii
  • ,
  • Kosei Takeuchi

4
開始ページ
190
終了ページ
203
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.isci.2018.05.019

Neuronal growth cones are essential for nerve growth and regeneration, as well as for the formation and rearrangement of the neural network. To elucidate phosphorylation-dependent signaling pathways and establish useful molecular markers for axon growth and regeneration, we performed a phosphoproteomics study of mammalian growth cones, which identified >30,000 phosphopeptides of ∼1,200 proteins. The phosphorylation sites were highly proline directed and primarily MAPK dependent, owing to the activation of JNK, suggesting that proteins that undergo proline-directed phosphorylation mediate nerve growth in the mammalian brain. Bioinformatics analysis revealed that phosphoproteins were enriched in microtubules and the cortical cytoskeleton. The most frequently phosphorylated site was S96 of GAP-43 (growth-associated protein 43-kDa), a vertebrate-specific protein involved in axon growth. This previously uncharacterized phosphorylation site was JNK dependent. S96 phosphorylation was specifically detected in growing and regenerating axons as the most frequent target of JNK signaling; thus it represents a promising new molecular marker for mammalian axonal growth and regeneration.

リンク情報
DOI
https://doi.org/10.1016/j.isci.2018.05.019
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30240740
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147025
ID情報
  • DOI : 10.1016/j.isci.2018.05.019
  • PubMed ID : 30240740
  • PubMed Central 記事ID : PMC6147025

エクスポート
BibTeX RIS