論文

査読有り 責任著者 国際誌
2022年7月11日

Measurement of autophagy via LC3 western blotting following DNA-damage-induced senescence.

STAR protocols
  • Hitomi Yamamoto-Imoto
  • ,
  • Eiji Hara
  • ,
  • Shuhei Nakamura
  • ,
  • Tamotsu Yoshimori

3
3
開始ページ
101539
終了ページ
101539
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.xpro.2022.101539

Senescent cells accumulation is associated with aging and age-related diseases, and recent findings suggest that autophagy, the activity of the intracellular degradation system, decreases during senescence. In this protocol, we detail steps to induce cellular senescence in response to DNA damage, evaluate the senescent state using SA-β-gal staining and western blot for p21, LAMP1, and Lamin B1, and detect autophagy via LC3 western blotting. This protocol can be used in most cell lines and for various types of senescent cells. For complete details on the use and execution of this protocol, please refer to Yamamoto-Imoto et al. (2022).

リンク情報
DOI
https://doi.org/10.1016/j.xpro.2022.101539
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/35819884
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283947
ID情報
  • DOI : 10.1016/j.xpro.2022.101539
  • PubMed ID : 35819884
  • PubMed Central 記事ID : PMC9283947

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