2017年6月
High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates
Neuron
- 巻
- 94
- 号
- 6
- 開始ページ
- 1085
- 終了ページ
- 1100
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.neuron.2017.05.017
- 出版者・発行元
- CELL PRESS
Subcellular resolution imaging of the whole brain and subsequent image analysis are prerequisites for understanding anatomical and functional brain networks. Here, we have developed a very high-speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which acquires high-resolution images of a whole mouse brain in a speed range comparable to that of light-sheet fluorescence microscopy. FAST enables complete visualization of the brain at a resolution sufficient to resolve all cells and their subcellular structures. FAST renders unbiased quantitative group comparisons of normal and disease model brain cells for the whole brain at a high spatial resolution. Furthermore, FAST is highly scalable to non-human primate brains and human postmortem brain tissues, and can visualize neuronal projections in a whole adult marmoset brain. Thus, FAST provides new opportunities for global approaches that will allow for a better understanding of brain systems in multiple animal models and in human diseases.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.neuron.2017.05.017
- ISSN : 0896-6273
- eISSN : 1097-4199
- PubMed ID : 28641108
- Web of Science ID : WOS:000403820200008