論文

査読有り
2017年6月

High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates

Neuron
  • Kaoru Seiriki
  • Atsushi Kasai
  • Takeshi Hashimoto
  • Wiebke Schulze
  • Misaki Niu
  • Shun Yamaguchi
  • Takanobu Nakazawa
  • Ken-ichi Inoue
  • Shiori Uezono
  • Masahiko Takada
  • Yuichiro Naka
  • Hisato Igarashi
  • Masato Tanuma
  • James A. Waschek
  • Yukio Ago
  • Kenji F. Tanaka
  • Atsuko Hayata-Takano
  • Kazuki Nagayasu
  • Norihito Shintani
  • Ryota Hashimoto
  • Yasuto Kunii
  • Mizuki Hino
  • Junya Matsumoto
  • Hirooki Yabe
  • Takeharu Nagai
  • Katsumasa Fujita
  • Toshio Matsuda
  • Kazuhiro Takuma
  • Akemichi Baba
  • Hitoshi Hashimoto
  • 全て表示

94
6
開始ページ
1085
終了ページ
1100
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.neuron.2017.05.017
出版者・発行元
CELL PRESS

Subcellular resolution imaging of the whole brain and subsequent image analysis are prerequisites for understanding anatomical and functional brain networks. Here, we have developed a very high-speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which acquires high-resolution images of a whole mouse brain in a speed range comparable to that of light-sheet fluorescence microscopy. FAST enables complete visualization of the brain at a resolution sufficient to resolve all cells and their subcellular structures. FAST renders unbiased quantitative group comparisons of normal and disease model brain cells for the whole brain at a high spatial resolution. Furthermore, FAST is highly scalable to non-human primate brains and human postmortem brain tissues, and can visualize neuronal projections in a whole adult marmoset brain. Thus, FAST provides new opportunities for global approaches that will allow for a better understanding of brain systems in multiple animal models and in human diseases.

リンク情報
DOI
https://doi.org/10.1016/j.neuron.2017.05.017
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28641108
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000403820200008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.neuron.2017.05.017
  • ISSN : 0896-6273
  • eISSN : 1097-4199
  • PubMed ID : 28641108
  • Web of Science ID : WOS:000403820200008

エクスポート
BibTeX RIS