論文

査読有り
2012年5月

Diversity and functional analysis of proteorhodopsin in marine Flavobacteria

ENVIRONMENTAL MICROBIOLOGY
  • Susumu Yoshizawa
  • ,
  • Akira Kawanabe
  • ,
  • Hiroyasu Ito
  • ,
  • Hideki Kandori
  • ,
  • Kazuhiro Kogure

14
5
開始ページ
1240
終了ページ
1248
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/j.1462-2920.2012.02702.x
出版者・発行元
WILEY-BLACKWELL

Proteorhodopsin (PR) genes are widely distributed among marine prokaryotes and functions as light-driven proton pump when expressed heterologously in Escherichia coli, suggesting that light energy passing through PR may be substantial in marine environment. However, there are no data on PR proton pump activities in native marine bacteria. Here, we demonstrate light-driven proton pump activity (c. 124 H+ PR-1 min-1) in recently isolated marine Flavobacteria. Among 75 isolates, 38 possessed the PR gene. Illumination of cell suspensions from all eight tested strains in five genera triggered marked pH drops. The action spectrum of proton pump activity closely matched the spectral distribution of the sea surface green light field. Addition of hydroxylamine to a solubilized membrane fraction shifted the spectrum to a form characteristic of PR photobleached into retinal oxime, indicating that PRs in flavobacterial cell membranes transform the photon dose in incident radiation into energy in the form of membrane potential. Our results revealed that PR-mediated proton transport can create the sufficient membrane potential for the ATP synthesis in native flavobacterial cells.


リンク情報
DOI
https://doi.org/10.1111/j.1462-2920.2012.02702.x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22329552
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000302934000011&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/j.1462-2920.2012.02702.x
  • ISSN : 1462-2912
  • PubMed ID : 22329552
  • Web of Science ID : WOS:000302934000011

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