論文

査読有り
2016年2月

Effects of unsaturated fatty acids on the kinetics of voltage-gated proton channels heterologously expressed in cultured cells

JOURNAL OF PHYSIOLOGY-LONDON
  • Akira Kawanabe
  • ,
  • Yasushi Okamura

594
3
開始ページ
595
終了ページ
610
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1113/JP271274
出版者・発行元
WILEY-BLACKWELL

Unsaturated fatty acids are key components of the biological membranes of all cells, and precursors of mediators for cell signalling. Arachidonic acid (AA) is an unsaturated fatty acid known to modulate the activities of various ion channels, including the voltage-gated proton (Hv) channel, which supports the rapid production of reactive oxygen species (ROS) in phagocytes through regulation of pH and membrane potential. However, the molecular mechanisms and physiological functions of the effects of AA on Hv channels remain unclear. In the present study, we report an electrophysiological analysis of the effects of AA on the mouse Hv channel (mHv1) heterologously expressed in HEK293T cells. Application of AA to excised inside-out patch membranes rapidly induced a robust increase in the amplitude of the proton current through mHv1. The current increase was accompanied by accelerated activation kinetics and a small leftward shift of the current-voltage relationship. In monomeric channels lacking the coiled-coil region of the channel protein, the shift in the current-voltage relationship was diminished but activation and deactivation remained accelerated. Studies with several AA derivatives showed that double bonds and hydrophilic head groups are essential for the effect of AA, although charge was not important. The application of phospholipase A(2) (PLA(2)), which generates AA from cell membrane phospholipids, stimulated mHv1 activity to a similar extent as direct application of approximate to 20m AA, suggesting that endogenous AA may regulate Hv channel activity.


リンク情報
DOI
https://doi.org/10.1113/JP271274
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000369860100006&DestApp=WOS_CPL
ID情報
  • DOI : 10.1113/JP271274
  • ISSN : 0022-3751
  • eISSN : 1469-7793
  • Web of Science ID : WOS:000369860100006

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