論文

査読有り
2016年12月

Comparison between mouse and sea urchin orthologs of voltage-gated proton channel suggests role of S3 segment in activation gating

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
  • Souhei Sakata
  • ,
  • Nana Miyawaki
  • ,
  • Thomas J. McCormack
  • ,
  • Hiroki Arima
  • ,
  • Akira Kawanabe
  • ,
  • Nurdan Oezkucur
  • ,
  • Tatsuki Kurokawa
  • ,
  • Yuka Jinno
  • ,
  • Yuichiro Fujiwara
  • ,
  • Yasushi Okamura

1858
12
開始ページ
2972
終了ページ
2983
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbamem.2016.09.008
出版者・発行元
ELSEVIER SCIENCE BV

The voltage-gated proton channel, Hv1, is expressed in blood cells, airway epithelium, sperm and microglia, playing important roles in diverse biological contexts including phagocytosis or sperm maturation through its regulation of membrane potential and pH. The gene encoding Hv1, HVCN1, is widely found across many species and is also conserved in unicellular organisms such as algae or dinoflagellates where Hv1 plays role in calcification or bioluminescence. Voltage-gated proton channels exhibit a large variation of activation rate among different species. Here we identify an Hv1 ortholog from sea urchin, Strongylocentrotus purpuratus, SpHv1. SpHv1 retains most of key properties of Hv1 but exhibits 20-60 times more rapid activation kinetics than mammalian orthologs upon heterologous expression in HEK293T cells. Comparison between SpHv1 and mHv1 highlights novel roles of the third transmembrane segment S3 in activation gating of Hv1. (C) 2016 Elsevier B.V. All tights reserved.


リンク情報
DOI
https://doi.org/10.1016/j.bbamem.2016.09.008
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000388048600003&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.bbamem.2016.09.008
  • ISSN : 0005-2736
  • eISSN : 0006-3002
  • Web of Science ID : WOS:000388048600003

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