論文

査読有り
2012年4月

Anomalous pH Effect of Blue Proteorhodopsin

JOURNAL OF PHYSICAL CHEMISTRY LETTERS
  • Keisuke Yamada
  • ,
  • Akira Kawanabe
  • ,
  • Susumu Yoshizawa
  • ,
  • Kentaro Inoue
  • ,
  • Kazuhiro Kogure
  • ,
  • Hideki Kandori

3
7
開始ページ
800
終了ページ
804
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/jz3000257
出版者・発行元
AMER CHEMICAL SOC

Proteorhodopsin (PR) is a light-driven proton pump found in marine bacteria, and thousands of PRs are classified into blue-absorbing PR (B-PR; lambda(max) approximate to 490 nm) and green-absorbing PR (G-PR; lambda(max) approximate to 525 nm). In this report, we present conversion of B-PR into G-PR using anomalous pH effect B-PR in LC1-200, marine gamma-proteobacteria, absorbs 497 and 513 nm maximally at pH 7 and 4, respectively, whose pH titration was reversible (pK(a) = 4.8). When pH was lowered from 4, the lambda(max) was further red-shifted (528 nm at pH 2). This is unusual because blue shift occurs by chloride binding in the case of bacteriorhodopsin. Surprisingly, when pH was increased from 2 to 7, the lambda(max) of this B-PR was further red-shifted to 540 nm, indicating that green-absorbing PR (PR540) is created only by changing pH. The present study reports the conformational flexibility of microbial rhodopsins, leading to the switch of absorbing color by a simple pH change.


リンク情報
DOI
https://doi.org/10.1021/jz3000257
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000302336800001&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-84859587597&partnerID=MN8TOARS
URL
http://orcid.org/0000-0001-9667-6010
ID情報
  • DOI : 10.1021/jz3000257
  • ISSN : 1948-7185
  • ORCIDのPut Code : 47220679
  • SCOPUS ID : 84859587597
  • Web of Science ID : WOS:000302336800001

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