2012年4月
Anomalous pH Effect of Blue Proteorhodopsin
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
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- 巻
- 3
- 号
- 7
- 開始ページ
- 800
- 終了ページ
- 804
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/jz3000257
- 出版者・発行元
- AMER CHEMICAL SOC
Proteorhodopsin (PR) is a light-driven proton pump found in marine bacteria, and thousands of PRs are classified into blue-absorbing PR (B-PR; lambda(max) approximate to 490 nm) and green-absorbing PR (G-PR; lambda(max) approximate to 525 nm). In this report, we present conversion of B-PR into G-PR using anomalous pH effect B-PR in LC1-200, marine gamma-proteobacteria, absorbs 497 and 513 nm maximally at pH 7 and 4, respectively, whose pH titration was reversible (pK(a) = 4.8). When pH was lowered from 4, the lambda(max) was further red-shifted (528 nm at pH 2). This is unusual because blue shift occurs by chloride binding in the case of bacteriorhodopsin. Surprisingly, when pH was increased from 2 to 7, the lambda(max) of this B-PR was further red-shifted to 540 nm, indicating that green-absorbing PR (PR540) is created only by changing pH. The present study reports the conformational flexibility of microbial rhodopsins, leading to the switch of absorbing color by a simple pH change.
- リンク情報
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- DOI
- https://doi.org/10.1021/jz3000257
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000302336800001&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-84859587597&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0001-9667-6010
- ID情報
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- DOI : 10.1021/jz3000257
- ISSN : 1948-7185
- ORCIDのPut Code : 47220679
- SCOPUS ID : 84859587597
- Web of Science ID : WOS:000302336800001