2016年11月29日
Erratum: Voltage-dependent motion of the catalytic region of voltage-sensing phosphatase monitored by a fluorescent amino acid (Proceedings of the National Academy of Sciences of the United States of America (2016) 113:27 (7521-7526) DOI: 10.1073/pnas.1604218113)
Proceedings of the National Academy of Sciences of the United States of America
- ,
- ,
- ,
- 巻
- 113
- 号
- 27
- 開始ページ
- 7521
- 終了ページ
- 7526
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1073/pnas.1604218113
- 出版者・発行元
- National Academy of Sciences
The cytoplasmic region of voltage-sensing phosphatase (VSP) derives the voltage dependence of its catalytic activity from coupling to a voltage sensor homologous to that of voltage-gated ion channels. To assess the conformational changes in the cytoplasmic region upon activation of the voltage sensor, we genetically incorporated a fluorescent unnatural amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap), into the catalytic region of Ciona intestinalis VSP (Ci-VSP). Measurements of Anap fluorescence under voltage clamp in Xenopus oocytes revealed that the catalytic region assumes distinct conformations dependent on the degree of voltage-sensor activation. FRET analysis showed that the catalytic region remains situated beneath the plasma membrane, irrespective of the voltage level. Moreover, Anap fluorescence from a membrane-facing loop in the C2 domain showed a pattern reflecting substrate turnover. These results indicate that the voltage sensor regulates Ci-VSP catalytic activity by causing conformational changes in the entire catalytic region, without changing their distance from the plasma membrane.
- ID情報
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- DOI : 10.1073/pnas.1604218113
- ISSN : 0027-8424
- ISSN : 1091-6490
- PubMed ID : 27330112
- SCOPUS ID : 84976571368