論文

査読有り 国際誌
2022年6月

Rhodopsin-bestrophin fusion proteins from unicellular algae form gigantic pentameric ion channels.

Nature structural & molecular biology
  • Andrey Rozenberg
  • ,
  • Igor Kaczmarczyk
  • ,
  • Donna Matzov
  • ,
  • Johannes Vierock
  • ,
  • Takashi Nagata
  • ,
  • Masahiro Sugiura
  • ,
  • Kota Katayama
  • ,
  • Yuma Kawasaki
  • ,
  • Masae Konno
  • ,
  • Yujiro Nagasaka
  • ,
  • Mako Aoyama
  • ,
  • Ishita Das
  • ,
  • Efrat Pahima
  • ,
  • Jonathan Church
  • ,
  • Suliman Adam
  • ,
  • Veniamin A Borin
  • ,
  • Ariel Chazan
  • ,
  • Sandra Augustin
  • ,
  • Jonas Wietek
  • ,
  • Julien Dine
  • ,
  • Yoav Peleg
  • ,
  • Akira Kawanabe
  • ,
  • Yuichiro Fujiwara
  • ,
  • Ofer Yizhar
  • ,
  • Mordechai Sheves
  • ,
  • Igor Schapiro
  • ,
  • Yuji Furutani
  • ,
  • Hideki Kandori
  • ,
  • Keiichi Inoue
  • ,
  • Peter Hegemann
  • ,
  • Oded Béjà
  • ,
  • Moran Shalev-Benami

29
6
開始ページ
592
終了ページ
603
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41594-022-00783-x

Many organisms sense light using rhodopsins, photoreceptive proteins containing a retinal chromophore. Here we report the discovery, structure and biophysical characterization of bestrhodopsins, a microbial rhodopsin subfamily from marine unicellular algae, in which one rhodopsin domain of eight transmembrane helices or, more often, two such domains in tandem, are C-terminally fused to a bestrophin channel. Cryo-EM analysis of a rhodopsin-rhodopsin-bestrophin fusion revealed that it forms a pentameric megacomplex (~700 kDa) with five rhodopsin pseudodimers surrounding the channel in the center. Bestrhodopsins are metastable and undergo photoconversion between red- and green-absorbing or green- and UVA-absorbing forms in the different variants. The retinal chromophore, in a unique binding pocket, photoisomerizes from all-trans to 11-cis form. Heterologously expressed bestrhodopsin behaves as a light-modulated anion channel.

リンク情報
DOI
https://doi.org/10.1038/s41594-022-00783-x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/35710843
ID情報
  • DOI : 10.1038/s41594-022-00783-x
  • PubMed ID : 35710843

エクスポート
BibTeX RIS