論文

査読有り 最終著者 責任著者 本文へのリンクあり 国際誌
2021年10月

Efficient messenger RNA delivery to the kidney using renal pelvis injection in mice

Pharmaceutics
  • Natsuko Oyama
  • ,
  • Maho Kawaguchi
  • ,
  • Keiji Itaka
  • ,
  • Shigeru Kawakami*

13
11
開始ページ
1810
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/pharmaceutics13111810

Renal dysfunction is often associated with the inflammatory cascade, leading to non-reversible nephrofibrosis. Gene therapy has the ability to treat the pathology. However, the difficulty in introducing genes into the kidney, via either viral vectors or plasmid DNA (pDNA), has hampered its extensive clinical use. Messenger RNA (mRNA) therapeutics has recently attracted attention as alternative gene therapies. mRNA allows protein production into post-mitotic cells without the need for transport to the nuclei in the target cells. However, few studies have reported the delivery of mRNA to the kidney. In this study, we attempted to deliver mRNA to the kidney based on the principle of pressure stimulation, by administering mRNA-loaded polyplex nanomicelles via a renal pelvis injection, directly into the kidney. Compared with the administration of naked plasmid DNA (pDNA) and naked mRNA, the mRNA-loaded nanomicelles diffusely induced protein expression in a greater number of cells at the tubular epithelium for some days. The plasma creatinine (Cre) and blood urea nitrogen (BUN) levels after the administration remained similar to those of the sham-operated controls, without marked changes in histological sections. The safety and efficacy of mRNA-loaded nanomicelles would make distinct contributions to the development of mRNA therapeutics for the kidney.

リンク情報
DOI
https://doi.org/10.3390/pharmaceutics13111810
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34834225
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619888
共同研究・競争的資金等の研究課題
mRNA送達を基盤とした革新的T細胞誘導型ワクチン医薬の創成
URL
https://www.mdpi.com/1999-4923/13/11/1810 本文へのリンクあり
ID情報
  • DOI : 10.3390/pharmaceutics13111810
  • PubMed ID : 34834225
  • PubMed Central 記事ID : PMC8619888

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