2016年12月19日
Minimization of cell-substrate interaction using suspended microstructured meshes initiates cell sheet formation by self-assembly organization
Biomedical Physics and Engineering Express
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- 巻
- 2
- 号
- 6
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1088/2057-1976/2/6/065019
- 出版者・発行元
- Institute of Physics Publishing
There is growing interest to achieve tissue fabrication by designing the architecture of the substrate. Here we present a mesh culture approach which enables fabrication of standalone cell sheets by cell-cell contact mediated self-assembly organizationon highly porous and microstructured SU-8 polymer meshes (apertures size >
100 μminlength, mesh strands 3-5 μminwidth), which are set suspended in a culture mediumto limit cell-substrate interaction only tothefine mesh strands. We demonstrate that ingression of cells into the wide mesh openings proceeds solely by cell-cell adhesion, resulting in the formationofauniform cell sheet over the highly porous mesh scaffolds. Auniformly spread actin meshwork dotted with reinforcement structures including transverse actin cables was present in cell sheets of fibroblasts while ring-like cables of actin/E-cadherin architecture was confirmed in epithelial cell sheets, suggesting the requirement for mechanically stable cell-cell adhesion architecture to overcome substrate adhesion limitations. Expression of type I collagen suggested the deposition of collagen and other extracellular matrices. Moreover, cell sheets could be maintained for an extended period of time (<
100 d) without noticeable loss of cell viability, owing to improved exposure of the cell layerto mediumina suspension setup. Thus, the mesh culture offers analternative method for extended cell culture and fabrication of standalone cell sheets for easy integration into microfluidics platforms for organ-on-chip studies.
100 μminlength, mesh strands 3-5 μminwidth), which are set suspended in a culture mediumto limit cell-substrate interaction only tothefine mesh strands. We demonstrate that ingression of cells into the wide mesh openings proceeds solely by cell-cell adhesion, resulting in the formationofauniform cell sheet over the highly porous mesh scaffolds. Auniformly spread actin meshwork dotted with reinforcement structures including transverse actin cables was present in cell sheets of fibroblasts while ring-like cables of actin/E-cadherin architecture was confirmed in epithelial cell sheets, suggesting the requirement for mechanically stable cell-cell adhesion architecture to overcome substrate adhesion limitations. Expression of type I collagen suggested the deposition of collagen and other extracellular matrices. Moreover, cell sheets could be maintained for an extended period of time (<
100 d) without noticeable loss of cell viability, owing to improved exposure of the cell layerto mediumina suspension setup. Thus, the mesh culture offers analternative method for extended cell culture and fabrication of standalone cell sheets for easy integration into microfluidics platforms for organ-on-chip studies.
- ID情報
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- DOI : 10.1088/2057-1976/2/6/065019
- ISSN : 2057-1976
- ORCIDのPut Code : 28930073
- SCOPUS ID : 85029350656