論文

2021年7月2日

Novel probiotic yeast from Miso promotes regulatory dendritic cell IL-10 production and attenuates DSS-induced colitis in mice.

Journal of gastroenterology
  • Yoshikiyo Okada
  • Yoshikazu Tsuzuki
  • Nao Sugihara
  • Shin Nishii
  • Naoki Shibuya
  • Akinori Mizoguchi
  • Suguru Itoh
  • Rina Tanemoto
  • Kenichi Inaba
  • Yoshinori Hanawa
  • Kazuki Horiuchi
  • Akinori Wada
  • Masaaki Higashiyama
  • Chikako Watanabe
  • Chie Kurihara
  • Shunsuke Komoto
  • Kengo Tomita
  • Soichiro Miura
  • Ryota Hokari
  • 全て表示

56
9
開始ページ
829
終了ページ
842
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00535-021-01804-0

BACKGROUND: Yeasts are a type of fungi thought to have probiotic functions. In this study, we isolated a novel probiotic yeast (Zygosaccharomyces sapae strain I-6) from Miso (a traditional Japanese fermented food). We examined its effects on phenotypic changes in intestinal dendritic cells (DCs), and evaluated its anti-inflammatory effects in dextran sulfate sodium (DSS)-induced colitis. METHODS: A single colony was selected from homogenized Miso, based on its ability to produce interleukin (IL)-10 in CD11c+ bone marrow DCs (BMDCs) in vitro. The anti-inflammatory effects of strain I-6 on CD11c+ BMDCs and CD11c+ CD103+ DCs were analyzed in mouse mesenteric lymph nodes in vitro and in a DSS mouse model. RESULTS: The IL-10 concentrations in the co-culture BMDC supernatants treated with I-6 were dramatically higher than in those treated with Saccharomyces cerevisiae (Sc). IL-10 production is mediated by both TLR2 and Dectin-1. β-Glucan extracted from I-6 also induced higher levels of IL-10 production in BMDCs than β-glucan from Sc. The number of mesenteric lymph node CD11c+ CD103+ DCs was significantly increased by I-6 administration, compared with Sc administration. Strain I-6 showed strong anti-inflammatory effects on DSS-induced colitis compared to Sc. Moreover, the adoptive transfer of I-6-treated BMDCs showed anti-inflammatory effects on DSS-induced colitis in mice without oral administration of I-6 cells. CONCLUSIONS: Strain I-6 induced phenotypic changes in intestinal CD11c+ DCs characterized by high IL-10 production and exerted strong anti-inflammatory effects on DSS-induced colitis. Traditional Japanese fermented foods may be a valuable source of probiotic yeasts for effective IBD therapy and treatment.

リンク情報
DOI
https://doi.org/10.1007/s00535-021-01804-0
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34213612
ID情報
  • DOI : 10.1007/s00535-021-01804-0
  • PubMed ID : 34213612

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