論文

査読有り
2016年12月1日

Measurement of amyloid formation by turbidity assay—seeing through the cloud

Biophysical Reviews
  • Ran Zhao
  • ,
  • Masatomo So
  • ,
  • Hendrik Maat
  • ,
  • Nicholas J. Ray
  • ,
  • Fumio Arisaka
  • ,
  • Yuji Goto
  • ,
  • John A. Carver
  • ,
  • Damien Hall

8
4
開始ページ
445
終了ページ
471
記述言語
英語
掲載種別
DOI
10.1007/s12551-016-0233-7
出版者・発行元
Springer Verlag

Detection of amyloid growth is commonly carried out by measurement of solution turbidity, a low-cost assay procedure based on the intrinsic light scattering properties of the protein aggregate. Here, we review the biophysical chemistry associated with the turbidimetric assay methodology, exploring the reviewed literature using a series of pedagogical kinetic simulations. In turn, these simulations are used to interrogate the literature concerned with in vitro drug screening and the assessment of amyloid aggregation mechanisms.

リンク情報
DOI
https://doi.org/10.1007/s12551-016-0233-7
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28003859

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