We have succeeded in controlling the hold and release of dye molecules using liquid crystalline nano-emulsions (LCNEs). In this paper, we studied the relation between the concentration of liquid crystalline mixture confined in the core of LCNEs and the phase transition temperature of the core part. Moreover, it was revealed that the release temperature, at which dye molecules are released from the core of LCNE, changes depending on the phase transition temperature of the core part of LCNEs. From these results, we will discuss the origin of hold and release of the dye molecules.