論文

査読有り
2018年4月1日

Quantitative determination of erlotinib in human serum using competitive enzyme-linked immunosorbent assay

Journal of Pharmaceutical Analysis
  • Yuta Yamamoto
  • ,
  • Tetsuya Saita
  • ,
  • Yutaro Yamamoto
  • ,
  • Masashi Shin

8
2
開始ページ
119
終了ページ
123
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jpha.2017.10.002
出版者・発行元
Xi'an Jiaotong University

A selective and sensitive competitive enzyme-linked immunosorbent assay (ELISA) method was developed and validated for the quantification of erlotinib in 50 µL of samples of human serum. Anti-erlotinib serum was obtained by immunizing mice with an antigen conjugated with bovine serum albumin and 3,4-bis(2-methoxyethoxy)benzoic acid using the N-succinimidyl ester method. Enzyme labeling of erlotinib with horseradish peroxidase was similarly performed using 3,4-bis(2-methoxyethoxy)benzoic acid. A simple competitive ELISA for erlotinib was developed using the principle of direct competition between erlotinib and the enzyme marker for anti-erlotinib antibody, which had been immobilized on the plastic surface of a microtiter plate. Serum erlotinib concentrations lower than 40 ng/mL were reproducibly measurable using the ELISA. This ELISA was specific to erlotinib and showed very slight cross-reactivity (6.7%) with a major metabolite, O-desmethyl erlotinib. Using this assay, drug levels were easily measured in the blood of mice after oral administration of erlotinib at a single dose of 30 mg/kg. ELISA should be used as a valuable tool for therapeutic drug monitoring and in pharmacokinetic studies of erlotinib.

リンク情報
DOI
https://doi.org/10.1016/j.jpha.2017.10.002
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29736298
ID情報
  • DOI : 10.1016/j.jpha.2017.10.002
  • ISSN : 2095-1779
  • PubMed ID : 29736298
  • SCOPUS ID : 85044925363

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