論文

査読有り
2015年11月

Quantification of Sorafenib in Human Serum by Competitive Enzyme-Linked Immunosorbent Assay

BIOLOGICAL & PHARMACEUTICAL BULLETIN
  • Tetsuya Saita
  • ,
  • Yuta Yamamoto
  • ,
  • Satoshi Noda
  • ,
  • Makoto Shioya
  • ,
  • Daiki Hira
  • ,
  • Akira Andoh
  • ,
  • Shin-ya Morita
  • ,
  • Tomohiro Terada
  • ,
  • Masashi Shin

38
11
開始ページ
1788
終了ページ
1793
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1248/bpb.b15-00484
出版者・発行元
PHARMACEUTICAL SOC JAPAN

The multikinase inhibitor sorafenib has been used in the treatment of hepatocellular carcinoma, renal cell carcinoma, and differentiated thyroid carcinoma. Here we have demonstrated the production of the first specific antibody against sorafenib. Anti-sorafenib serum was obtained by immunizing mice with an antigen conjugated with bovine serum albumin and carboxylic modified 4-(4-aminophenoxy)-N-methyl-2-pyridine-carboxamide (AMPC) using the N-succinimidyl ester method. Enzyme labeling of sorafenib with horseradish peroxidase was similarly performed using carboxylic modified AMPC. A simple competitive enzyme-linked immunosorbent assay (ELISA) for sorafenib was developed using the principle of direct competition between sorafenib and the enzyme marker for anti-sorafenib antibody, which had been adsorbed by the plastic surface of a microtiter plate. Serum sorafenib concentrations lower than 0.04 mu g/mL were reproducibly measurable using the ELISA. This ELISA was specific to sorafenib and showed very slight cross-reactivity (2.5%) with a major metabolite, sorafenib N-oxide. The values of serum sorafenib levels from 32 patients measured by this ELISA were comparable with those measured by HPLC, and there was a strong correlation between the values determined by the two methods (Y=1.016X-0.137, r=0.979). The specificity and sensitivity of the ELISA for sorafenib should provide a valuable new tool for use in therapeutic drug monitoring and pharmacokinetic studies of sorafenib.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1248/bpb.b15-00484
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26521829
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000363907600015&DestApp=WOS_CPL
ID情報
  • DOI : 10.1248/bpb.b15-00484
  • ISSN : 0918-6158
  • PubMed ID : 26521829
  • Web of Science ID : WOS:000363907600015

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