論文

査読有り
2015年10月

Preparation of Antibodies and Development of an Enzyme-Linked Immunosorbent Assay for the Tyrosine Kinase Inhibitors Lapatinib and Nilotinib

BIOLOGICAL & PHARMACEUTICAL BULLETIN
  • Tetsuya Saita
  • ,
  • Yuta Yamamoto
  • ,
  • Masashi Shin
  • ,
  • Yukitaka Nakano

38
10
開始ページ
1652
終了ページ
1657
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1248/bpb.b15-00330
出版者・発行元
PHARMACEUTICAL SOC JAPAN

In this paper, we describe the production of the first specific antibodies against the tyrosine kinase inhibitors lapatinib and nilotinib. Anti-lapatinib antibody was obtained by immunizing rabbits with an antigen conjugated with bovine serum albumin using 3-chloro-4((3-fluorobenzyl)oxy)aniline. Anti-nilotinib antibody was produced by immunizing mice with an antigen conjugated with bovine serum albumin using 2-(5-amino-2-methylanilino)-4-(3-pyridyl)pyrimidine. The generated antibodies were used to develop highly sensitive and specific enzyme-linked immunosorbent assays (ELISAs) for lapatinib and nilotinib in human serum. The assays were capable of detecting lapatinib and nilotinib at serum concentrations as low as 40 and 8 ng/mL, respectively. Using the two ELISAs, drugs levels were easily measured in the serum of rats after a single dose oral administration of lapatinib or nilotinib. The assays are therefore expected be valuable tools for therapeutic drug monitoring in the clinical setting and pharmacokinetic studies of lapatinib and nilotinib.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1248/bpb.b15-00330
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26424026
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000362047200028&DestApp=WOS_CPL
ID情報
  • DOI : 10.1248/bpb.b15-00330
  • ISSN : 0918-6158
  • PubMed ID : 26424026
  • Web of Science ID : WOS:000362047200028

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