論文

査読有り
2012年6月

Development of a Specific and Sensitive Enzyme-linked Immunosorbent Assay for Vindesine

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN
  • Yukitaka Nakano
  • ,
  • Tetsuya Saita
  • ,
  • Hiroshi Fujito

132
6
開始ページ
727
終了ページ
732
記述言語
日本語
掲載種別
研究論文(学術雑誌)
出版者・発行元
PHARMACEUTICAL SOC JAPAN

This paper reports a specific and sensitive enzyme-linked immunosorbent assay (ELISA) for pharmacokinetic studies of vindesine (VDS). Anti-VDS antibody was obtained by immunizing rabbits with VDS conjugated with bovine serum albumin using N-[beta-(4-diazophenyl) ethyl] maleimide as a heterobifunctional coupling agent. An enzyme marker was similarly prepared by coupling VDS with horseradish peroxidase using N-(4-diazophenyl) maleimide. The detection limit of VDS by ELISA was approximately 24 pg/mL with 50-mL samples. This assay was specific for VDS and showed very slight cross-reactivity with other vinca alkaloids, vincristine (0.18%) and vinblastine (0.11%). The values for the VDS concentrations detected using this assay were comparable with those detected using HPLC. There was a good correlation between the values determined by the two methods. Moreover, ELISA was about 50-fold more sensitive in detecting VDS at lower concentrations. The sensitivity and specificity of ELISA should provide a useful tool for pharmacokinetic studies of VDS.

Web of Science ® 被引用回数 : 3

リンク情報
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22687732
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000304724700007&DestApp=WOS_CPL
ID情報
  • ISSN : 0031-6903
  • PubMed ID : 22687732
  • Web of Science ID : WOS:000304724700007

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