論文

査読有り
2002年8月

Development of an enzyme-linked immunosorbent assay for the quantification of amiodarone

BIOLOGICAL & PHARMACEUTICAL BULLETIN
  • T Saita
  • ,
  • H Fujito
  • ,
  • M Mori

25
8
開始ページ
954
終了ページ
958
記述言語
英語
掲載種別
研究論文(学術雑誌)
出版者・発行元
PHARMACEUTICAL SOC JAPAN

A sensitive and specific enzyme-linked immunosorbent assay for an antiarrhythmic drug, amiodarone (AMI), was developed, which is capable of measuring levels as low as 16 ng/ml. Anti-AMI antibody was obtained by immunizing rabbits with an antigen conjugated with bovine serum albumin using diazotized 4-amino-1-(2-diethylaminoethoxy)-2,6-diiodobenzene. Enzyme labeling of AMI with beta-D-galactosidase was similarly performed using a diazotized 4-amino-1-(2-diethylaminoethoxy)-2,6-diiodobenzene. This enzyme-linked immunosorbent assay was specific for AMI and showed a very slight cross-reactivity (1.25%) with its major metabolite, mono-N-desethylamiodarone. The values of the AMI concentrations measured by this assay were in good correlation to those by HPLC. Its analytical applicability was demonstrated by a kinetic study with human liver microsomes. The enzyme-linked immunosorbent assay should be a valuable tool in therapeutic drug monitoring and pharmacokinetic studies of AMI.

Web of Science ® 被引用回数 : 6

リンク情報
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/12186425
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000177198000002&DestApp=WOS_CPL
ID情報
  • ISSN : 0918-6158
  • PubMed ID : 12186425
  • Web of Science ID : WOS:000177198000002

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