論文

査読有り
2002年7月

Liquid chromatographic determination of triethylenetetramine in human and rabbit sera based on intramolecular excimer-forming fluorescence derivatization

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
  • Y Nakano
  • ,
  • H Nohta
  • ,
  • H Yoshida
  • ,
  • T Saita
  • ,
  • H Fujito
  • ,
  • M Mori
  • ,
  • M Yamaguchi

774
2
開始ページ
165
終了ページ
172
記述言語
英語
掲載種別
研究論文(学術雑誌)
出版者・発行元
ELSEVIER SCIENCE BV

A highly selective and simple fluorimetric liquid chromatographic method for the determination of triethylenetetramine (TETA), a therapeutic drug for Wilson's disease, in human and rabbit sera is described. This method is based on intramolecular excimer-forming fluorescence derivatization, which allows spectrofluorometric discrimination of polyamino compounds from monoamino species, followed by liquid chromatography. TETA and 1,6-hexanediamine (internal standard) were converted to the corresponding excimer-forming derivatives with a pyrene reagent, 4-(1-pyrene)butyric acid N-hydroxysuccinimide ester. The derivatives were separated within 20 min on a reversed-phase column using isocratic elution and detected spectofluorometrically at 480 nm with excitation at 345 nm. This method was successfully applied to the monitoring of TETA in human and rabbit sera with a simple pretreatment. The detection limit for TETA in serum was 18 ng/ml (0.13 nmol/ml) corresponding to 0.2 pmol on column at a signal-to-noise ratio of 3. (C) 2002 Elsevier Science B.V. All rights reserved.

Web of Science ® 被引用回数 : 27

リンク情報
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/12076686
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000176996300005&DestApp=WOS_CPL
ID情報
  • ISSN : 1570-0232
  • PubMed ID : 12076686
  • Web of Science ID : WOS:000176996300005

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