論文

査読有り
2017年8月

A microsensing system for the in vivo real-time detection of local drug kinetics

NATURE BIOMEDICAL ENGINEERING
  • Genki Ogata
  • ,
  • Yuya Ishii
  • ,
  • Kai Asai
  • ,
  • Yamato Sano
  • ,
  • Fumiaki Nin
  • ,
  • Takamasa Yoshida
  • ,
  • Taiga Higuchi
  • ,
  • Seishiro Sawamura
  • ,
  • Takeru Ota
  • ,
  • Karin Hori
  • ,
  • Kazuya Maeda
  • ,
  • Shizuo Komune
  • ,
  • Katsumi Doi
  • ,
  • Madoka Takai
  • ,
  • Ian Findlay
  • ,
  • Hiroyuki Kusuhara
  • ,
  • Yasuaki Einaga
  • ,
  • Hiroshi Hibino

1
8
開始ページ
654
終了ページ
666
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41551-017-0118-5
出版者・発行元
NATURE PUBLISHING GROUP

Real-time recording of the kinetics of systemically administered drugs in in vivo microenvironments may accelerate the development of effective medical therapies. However, conventional methods require considerable analyte quantities, have low sampling rates and do not address how drug kinetics correlate with target function over time. Here, we describe the development and application of a drug-sensing system consisting of a glass microelectrode and a microsensor composed of boron-doped diamond with a tip of around 40 mu m in diameter. We show that, in the guinea pig cochlea, the system can measure-simultaneously and in real time-changes in the concentration of bumetanide (a diuretic that is ototoxic but applicable to epilepsy treatment) and the endocochlear potential underlying hearing. In the rat brain, we tracked the kinetics of the drug and the local field potentials representing neuronal activity. We also show that the actions of the antiepileptic drug lamotrigine and the anticancer reagent doxorubicin can be monitored in vivo. Our microsensing system offers the potential to detect pharmacological and physiological responses that might otherwise remain undetected.

Web of Science ® 被引用回数 : 20

リンク情報
DOI
https://doi.org/10.1038/s41551-017-0118-5
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000418858400004&DestApp=WOS_CPL

エクスポート
BibTeX RIS