論文

査読有り
2016年7月

Locomotor improvement of spinal cord-injured rats through treadmill training by forced plantar placement of hind paws

SPINAL CORD
  • M. Hayashibe
  • ,
  • T. Homma
  • ,
  • K. Fujimoto
  • ,
  • T. Oi
  • ,
  • N. Yagi
  • ,
  • M. Kashihara
  • ,
  • N. Nishikawa
  • ,
  • Y. Ishizumi
  • ,
  • S. Abe
  • ,
  • H. Hashimoto
  • ,
  • K. Kanekiyo
  • ,
  • H. Imagita
  • ,
  • C. Ide
  • ,
  • S. Morioka

54
7
開始ページ
521
終了ページ
529
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/sc.2015.186
出版者・発行元
NATURE PUBLISHING GROUP

Study design: Experimental training model of rats with spinal cord injury (SCI).
Setting: Osaka, Japan
Objective: To investigate the effect of forced treadmill training by plantar placement (PP), as compared with dorsal placement (DP), of the dorsal paws on the locomotor behaviors of spinal cord-injured rats.
Methods: The spinal cord was contusion-injured at the thoracic level. Rats were divided into three groups: forced training involving stepping by PP and DP and non-forced training/assistance (nT). Training began 1 week after injury and was conducted for 4 weeks. Locomotor behaviors were estimated using Basso-Beattie-Bresnahan (BBB) scores, dorsiflexion of the hind paws and footprints of the hind paws. Histological and immunohistochemical examinations of the spinal cord lesions were conducted after 4 weeks of training.
Results: The values, respectively, of PP, DP and nT groups at 4 weeks of training were as follows: BBB scores were 15.6 +/- 0.8, 7.7 +/- 1.3 and 10.3 +/- 0.4. The paw dorsiflexion angles were 34.1 +/- 5.2, 16.4 +/- 2.4 and 23.6 +/- 3.0 degrees, respectively. The stride angles were 5.1 +/- 0.9, 13.7 +/- 4.9 and 17.8 +/- 4.0 degrees for the left paws. Cavity volumes were 10.3 +/- 2.1, 31.0 +/- 2.0 and 28.2 +/- 4.9%. In addition to cavities, there were astrocyte-devoid areas containing some loose tissues, through which many axons extended longitudinally.
Conclusions: The BBB score, dorsiflexion angle and stride angle were consistently improved in the PP group. Cavity formation was more reduced, and many axons extended through coarse tissues formed in astrocyte-devoid areas at the lesion in the PP group. Forced training by PP of the hind paws promoted the behavioral and histological improvement of rats with SCI.

Web of Science ® 被引用回数 : 17

リンク情報
DOI
https://doi.org/10.1038/sc.2015.186
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000378872700006&DestApp=WOS_CPL
URL
https://www.ncbi.nlm.nih.gov/pubmed/?term=Locomotor+improvement+of+spinal+cord-injured+rats+through+treadmill+training+by+forced+plantar+placement+of+hind+paws.
ID情報
  • DOI : 10.1038/sc.2015.186
  • ISSN : 1362-4393
  • eISSN : 1476-5624
  • Web of Science ID : WOS:000378872700006

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