2022年6月17日
Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media.
STAR Protocols
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- 巻
- 3
- 号
- 2
- 開始ページ
- 101341
- 終了ページ
- 101341
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.xpro.2022.101341
We describe a protocol for the efficient culture of human pluripotent stem cells (hPSCs) by supplementing conventional culture medium with L-tryptophan (TRP). TRP is an essential amino acid that is widely available at an affordable cost, thereby allowing cost-effective proliferation of hPSCs compared to using a conventional medium alone. Here, we describe the steps for enhanced proliferation of hPSCs from dermal fibroblasts or peripheral blood cells, but the protocol can be applied to any hPSCs. For complete details on the use and execution of this protocol, please refer to Someya et al. (2021).
- リンク情報
- ID情報
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- DOI : 10.1016/j.xpro.2022.101341
- PubMed ID : 35496810
- PubMed Central 記事ID : PMC9048135