論文

査読有り
2019年5月

SOCS3 overexpression in T cells ameliorates chronic airway obstruction in a murine heterotopic tracheal transplantation model.

Surgery today
  • Kumi Mesaki
  • ,
  • Masaomi Yamane
  • ,
  • Seiichiro Sugimoto
  • ,
  • Masayoshi Fujisawa
  • ,
  • Teizo Yoshimura
  • ,
  • Takeshi Kurosaki
  • ,
  • Shinji Otani
  • ,
  • Shinichiro Miyoshi
  • ,
  • Takahiro Oto
  • ,
  • Akihiro Matsukawa
  • ,
  • Shinichi Toyooka

49
5
開始ページ
443
終了ページ
450
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00595-018-1753-5

PURPOSE: Suppressor of cytokine signaling-3 (SOCS3) is a negative feedback inhibitor of cytokine signaling with T-cell-mediated immunosuppressive effects on obliterative bronchiolitis (OB). In this study, we aimed to investigate the impact of T-cell-specific overexpression of SOCS3 using a murine heterotopic tracheal transplantation (HTT) model. METHODS: Tracheal allografts from BALB/c mice were subcutaneously transplanted into wild-type C57BL/6J (B6; WT) mice and SOCS3 transgenic B6 (SOCS3TG) mice. Tracheal allografts were analyzed by immunohistochemistry and quantitative polymerase chain reaction assays at days 7 and 21. RESULTS: At day 21, allografts in SOCS3TG mice showed significant amelioration of airway obstruction and epithelial loss compared with allografts in WT mice. The intragraft expression of IFN-γ and CXCL10 was suppressed, while that of IL-4 was enhanced in SOCS3TG mice at day 7. The T-bet levels were lower in SOCS3TG allografts than in WT allografts at day 7. CONCLUSION: We revealed that the overexpression of SOCS3 in T cells effectively ameliorates OB development in a murine HTT model by inhibiting the Th1 phenotype in the early phase. Our results suggest that the regulation of the T-cell response, through the modulation of SOCS expression, has potential as a new therapeutic strategy for chronic lung allograft dysfunction.

リンク情報
DOI
https://doi.org/10.1007/s00595-018-1753-5
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30617600
ID情報
  • DOI : 10.1007/s00595-018-1753-5
  • ISSN : 0941-1291
  • PubMed ID : 30617600

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