論文

査読有り 国際誌
2018年11月

Comparative mutational evaluation of multiple lung cancers by multiplex oncogene mutation analysis.

Cancer science
  • Yuta Takahashi
  • ,
  • Kazuhiko Shien
  • ,
  • Shuta Tomida
  • ,
  • Shinsuke Oda
  • ,
  • Takehiro Matsubara
  • ,
  • Hiroki Sato
  • ,
  • Ken Suzawa
  • ,
  • Eisuke Kurihara
  • ,
  • Yusuke Ogoshi
  • ,
  • Kei Namba
  • ,
  • Takahiro Yoshioka
  • ,
  • Hidejiro Torigoe
  • ,
  • Hiromasa Yamamoto
  • ,
  • Junichi Soh
  • ,
  • Shinichi Toyooka

109
11
開始ページ
3634
終了ページ
3642
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/cas.13797

In patients presenting with synchronous or metachronous multiple lung cancer (MLC), it is important to distinguish between multiple primary lung cancer (MP) and intrapulmonary metastasis (IM). The present study was aimed at investigating the mutational profiles of synchronous/metachronous MLC and to compare the classification of paired tumors by multiplex gene mutation analysis with the histopathological evaluation. We carried out targeted sequencing of 20 lung cancer-related oncogenes using next-generation sequencing (NGS) in 82 tumors from 37 MLC patients who underwent surgical resection at our department. The patients were diagnosed as MP or IM cases based on the Martini and Melamed criteria, histopathological and gene mutational evaluations. Matching mutations between paired tumors was observed in 20 (54%) patients, who were diagnosed as IM cases by mutational evaluation. Patients who could not be clearly diagnosed by histopathological evaluation were classified as equivocal cases. Among the histopathological IM cases (n = 7), six (86%) were confirmed as IM cases also by mutational evaluation, and most of the paired tumors of these cases (n = 5) harbored multiple matching mutations. Among the histopathological MP cases (n = 17), mutational evaluation yielded a discordant diagnosis in eight (47%) cases. Of these, the paired tumors of four cases harbored multiple matching mutations, suggesting that the mutational diagnosis might be more suitable in these patients. Our findings suggest that multiplex mutational analysis could be a useful complementary tool for distinguishing between MP and IM in addition to histopathological evaluation.

リンク情報
DOI
https://doi.org/10.1111/cas.13797
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30216592
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215894
ID情報
  • DOI : 10.1111/cas.13797
  • ISSN : 1347-9032
  • PubMed ID : 30216592
  • PubMed Central 記事ID : PMC6215894

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