論文

査読有り
2015年10月

Downregulation of TBXAS1 in an iron-induced malignant mesothelioma model

CANCER SCIENCE
  • Daisuke Minami
  • ,
  • Nagio Takigawa
  • ,
  • Yuka Kato
  • ,
  • Kenichiro Kudo
  • ,
  • Hideko Isozaki
  • ,
  • Shinsuke Hashida
  • ,
  • Daijiro Harada
  • ,
  • Nobuaki Ochi
  • ,
  • Masanori Fujii
  • ,
  • Toshio Kubo
  • ,
  • Kadoaki Ohashi
  • ,
  • Akiko Sato
  • ,
  • Takehiro Tanaka
  • ,
  • Katsuyuki Hotta
  • ,
  • Masahiro Tabata
  • ,
  • Shinichi Toyooka
  • ,
  • Mitsune Tanimoto
  • ,
  • Katsuyuki Kiura

106
10
開始ページ
1296
終了ページ
1302
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/cas.12752
出版者・発行元
WILEY-BLACKWELL

Malignant mesothelioma is an aggressive and therapy-resistant neoplasm arising from mesothelial cells. Evidence suggests that the major pathology associated with asbestos-induced mesothelioma is local iron overload. In the present study, we induced iron-induced mesothelioma in rats based on previous reports. Ten Wistar rats were given ferric saccharate and nitrilotriacetate i.p. for 5 days a week. Five of the ten rats exhibited widespread mesotheliomas in the peritoneum and tunica vaginalis. The tumor cells showed positive immunostaining for calretinin, wilms tumor-1, podoplanin and the oxidative DNA marker 8-hydroxy-2-deoxyguanosine. In three of the five rats with mesothelioma, array-based comparative genomic hybridization analysis identified a common chromosomal deletion mapped to the chromosomal 4q31 locus, which encompasses the TBXAS1 gene. Downregulation of the TBXAS1 gene was confirmed using quantitative PCR. TBXAS1 gene expression was also reduced in three of four human malignant pleural mesothelioma cell lines compared with normal bronchial epithelial cells. Immunohistochemistry revealed that TBXAS1 expression was weakly positive and positive in five and three out of eight human malignant mesothelioma samples, respectively. In conclusion, TBXAS1 gene expression was downregulated in rats with iron-induced mesothelioma. The relationship between iron overload and TBXAS1 downregulation should be pursued further.

Web of Science ® 被引用回数 : 7

リンク情報
DOI
https://doi.org/10.1111/cas.12752
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26211743
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000363731300006&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/cas.12752
  • ISSN : 1347-9032
  • eISSN : 1349-7006
  • PubMed ID : 26211743
  • Web of Science ID : WOS:000363731300006

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