論文

査読有り
2013年12月

The degree of microRNA-34b/c methylation in serum-circulating DNA is associated with malignant pleural mesothelioma

LUNG CANCER
  • Takayuki Muraoka
  • Junichi Soh
  • Shinichi Toyooka
  • Keisuke Aoe
  • Nobukazu Fujimoto
  • Shinsuke Hashida
  • Yuho Maki
  • Norimitsu Tanaka
  • Kazuhiko Shien
  • Masashi Furukawa
  • Hiromasa Yamamoto
  • Hiroaki Asano
  • Kazunori Tsukuda
  • Takumi Kishimoto
  • Takemi Otsuki
  • Shinichiro Miyoshi
  • 全て表示

82
3
開始ページ
485
終了ページ
490
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.lungcan.2013.09.017
出版者・発行元
ELSEVIER IRELAND LTD

Objectives: Malignant pleural mesothelioma (MPM) is an aggressive tumor with a poor prognosis. microRNA-34b/c (miR-34b/c), which plays an important role in the pathogenesis of MPM, is frequently downregulated by DNA methylation in approximately 90% of MPM cases. In this study, we estimated the degree of miR-34b/c methylation in serum-circulating DNA using a digital methylation specific PCR assay (MSP).
Materials and methods: A real-time MSP assay was performed using the SYBR Green method. The melting temperature (Tm) of each PCR product was examined using a melting curve analysis. For a digital MSP assay, 40 wells were analyzed per sample. A total of 110 serum samples from 48 MPM cases, 21 benign asbestos pleurisy (BAP) cases, and 41 healthy volunteers (HVs) were examined.
Results: Positive range of Tm value for miR-34b/c methylation was defined as 77.71-78.79 degrees C which was the mean 3 standard deviations of 40 wells of a positive control. The number of miR-34b/c methylated wells was counted per sample according to this criterion. The number of miR-34b/c methylated wells in MPM cases was significantly higher than that in BAP cases (P = 0.03) or HVs (P < 0.001). Advanced MPM cases tended to have higher number of miR-34b/c methylated wells than early MPM cases. Receiver-operating characteristic (ROC) curve analysis revealed that three number of miR-34b/c methylated wells per sample was the best cut-off of positivity of MPM with a 67% of sensitivity and a 77% specificity for prediction. The area under the ROC curve was 0.77.
Conclusions: Our digital MSP assay can quantify miR-34b/c methylation in serum-circulating DNA. The degree of miR-34b/c methylation in serum-circulating DNA is associated with MPM, suggesting that this approach might be useful for the establishment of a new detection system for MPM. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.lungcan.2013.09.017
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24168922
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000328181600017&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.lungcan.2013.09.017
  • ISSN : 0169-5002
  • eISSN : 1872-8332
  • PubMed ID : 24168922
  • Web of Science ID : WOS:000328181600017

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