論文

査読有り
2018年8月20日

Oocyte-specific gene Oog1 suppresses the expression of spermatogenesis-specific genes in oocytes.

The Journal of reproduction and development
  • Shinnosuke Honda
  • ,
  • Yuka Miki
  • ,
  • Yuya Miyamoto
  • ,
  • Yu Kawahara
  • ,
  • Satoshi Tsukamoto
  • ,
  • Hiroshi Imai
  • ,
  • Naojiro Minami

64
4
開始ページ
297
終了ページ
301
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1262/jrd.2018-024

Oog1, an oocyte-specific gene that encodes a protein of 425 amino acids, is present in five copies on mouse chromosomes 4 and 12. In mouse oocytes, Oog1 mRNA expression begins at embryonic day 15.5 and almost disappears by the late two-cell stage. Meanwhile, OOG1 protein is detectable in oocytes in ovarian cysts and disappears by the four-cell stage; the protein is transported to the nucleus in late one-cell to early two-cell stage embryos. In this study, we examined the role of Oog1 during oogenesis in mice. Oog1 RNAi-transgenic mice were generated by expressing double-stranded hairpin Oog1 RNA, which is processed into siRNAs targeting Oog1 mRNA. Quantitative RT-PCR revealed that the amount of Oog1 mRNA was dramatically reduced in oocytes obtained from Oog1-knockdown mice, whereas the abundance of spermatogenesis-associated transcripts (Klhl10, Tekt2, Tdrd6, and Tnp2) was increased in Oog1 knockdown ovaries. Tdrd6 is involved in the formation of the chromatoid body, Tnp2 contributes to the formation of sperm heads, Tekt2 is required for the formation of ciliary and flagellar microtubules, and Klhl10 plays a key role in the elongated sperm differentiation. These results indicate that Oog1 down-regulates the expression of spermatogenesis-associated genes in female germ cells, allowing them to develop normally into oocytes.

リンク情報
DOI
https://doi.org/10.1262/jrd.2018-024
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29731491
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6105735

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