論文

査読有り 筆頭著者 国際誌
2009年7月

The Cytoplasmic Tail of GM3 Synthase Defines Its Subcellular Localization, Stability, and In Vivo Activity

MOLECULAR BIOLOGY OF THE CELL
  • Satoshi Uemura
  • ,
  • Sayaka Yoshida
  • ,
  • Fumi Shishido
  • ,
  • Jin-ichi Inokuchi

20
13
開始ページ
3088
終了ページ
3100
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1091/mbc.E08-12-1219
出版者・発行元
AMER SOC CELL BIOLOGY

GM3 synthase (SAT-I) is the primary glycosyltransferase responsible for the biosynthesis of ganglio-series gangliosides. In this study, we identify three isoforms of mouse SAT-I proteins, named M1-SAT-I, M2-SAT-I, and M3-SAT-I, which possess distinct lengths in their NH(2)-terminal cytoplasmic tails. These isoforms are produced by leaky scanning from mRNA variants of mSAT-Ia and mSAT-Ib. M2-SAT-I and M3-SAT-I were found to be localized in the Golgi apparatus, as expected, whereas M1-SAT-I was exclusively found in the endoplasmic reticulum (ER). Specific multiple arginines (R) arranged in an R-based motif, RRXXXXR necessary for ER targeting, were found in the cytoplasmic tail of M1-SAT-I, and in vivo GM3 biosynthesis by M1-SAT-I was very low because of restricted transport to the Golgi apparatus. In addition, M1-SAT-I and M3-SAT-I had a long half-life relative to M2-SAT-I. This is the first report demonstrating the presence of an ER-targeting R-based motif in the cytoplasmic tail of a protein in the mammalian glycosyltransferase family of enzymes. The system, which produces SAT-I isoforms having distinct characteristics, is likely to be of critical importance for the regulation of GM3 biosynthesis under various pathological and physiological conditions.

リンク情報
DOI
https://doi.org/10.1091/mbc.E08-12-1219
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19420140
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000267536400009&DestApp=WOS_CPL
ID情報
  • DOI : 10.1091/mbc.E08-12-1219
  • ISSN : 1059-1524
  • PubMed ID : 19420140
  • Web of Science ID : WOS:000267536400009

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