© 2019 by the authors. Recently, deep learning technology has been applied to medical images. This study aimed to create a detector able to automatically detect an anatomical structure presented in a brain magnetic resonance imaging (MRI) scan to draw a standard line. A total of 1200 brain sagittal MRI scans were used for training and validation. Two sizes of regions of interest (ROIs) were drawn on each anatomical structure measuring 64 × 64 pixels and 32 × 32 pixels, respectively. Data augmentation was applied to these ROIs. The faster region-based convolutional neural network was used as the network model for training. The detectors created were validated to evaluate the precision of detection. Anatomical structures detected by the model created were processed to draw the standard line. The average precision of anatomical detection, detection rate of the standard line, and accuracy rate of achieving a correct drawing were evaluated. For the 64 × 64-pixel ROI, the mean average precision achieved a result of 0.76 ± 0.04, which was higher than the outcome achieved with the 32 × 32-pixel ROI. Moreover, the detection and accuracy rates of the angle of difference at 10 degrees for the orbitomeatal line were 93.3 ± 5.2 and 76.7 ± 11.0, respectively. The automatic detection of a reference line for brain MRI can help technologists improve this examination.