2009年1月
Comparative study of antioxidant activity and antiproliferative effect of hot water and ethanol extracts from the mushroom Inonotus obliquus
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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- 巻
- 107
- 号
- 1
- 開始ページ
- 42
- 終了ページ
- 48
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.jbiosc.2008.09.004
- 出版者・発行元
- SOC BIOSCIENCE BIOENGINEERING JAPAN
The medicinal mushroom Inonotus obliquus is a traditional and widely used multi-functional fungus. Hot water (50 degrees C, 70 degrees C, and 80 degrees C) and ethanol crude extracts of L obliquus were investigated for their antioxidant activity with superoxide dismutase (SOD) and (1,1-diphenyl-2-picryhydrazyl) (DPPH) radical-scavenging activity assays. We also investigated the antiproliferative effects and ability of the extracts to induce apoptosis in human colon cancer DLD-1 cells. Among the four extracts, the ethanol extract (EE) exhibited the strongest SOD-like activity and antiproliferative effect on DLD-1 cells, and exposure to the EE resulted in the induction of apoptosis, whereas no apoptosis was observed in DLD-1 cells exposed to the hot water extracts (HWEs). HWE at 70 degrees C (HWE70) exhibited the strongest DPPH radical-scavenging activity (EC50,126 mu g/ml), whereas the EE showed the weakest activity (EC50, 224 mu g/ml). The different biological activities among the four extracts may be attributed to differences in their chemical composition, partially supported by polysaccharide, protein and phenolic content, and the (1)H-NMR spectra. (C) 2008, The Society for Biotechnology, Japan. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.jbiosc.2008.09.004
- CiNii Articles
- http://ci.nii.ac.jp/naid/110007042034
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/19147108
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000263531300009&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.jbiosc.2008.09.004
- ISSN : 1389-1723
- CiNii Articles ID : 110007042034
- PubMed ID : 19147108
- Web of Science ID : WOS:000263531300009